Recombinant Bovine Arrestin-C (ARR3)

Code CSB-YP878783BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP878783BO
MSDS
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Source E.coli
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Code CSB-EP878783BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878783BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP878783BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARR3
Uniprot No.
Alternative Names
ARR3Arrestin-C; Cone arrestin; cArr; Retinal cone arrestin-3; X-arrestin
Species
Bos taurus (Bovine)
Expression Region
1-389
Target Protein Sequence
MSRVFKKTCS NGKLSIYLGK RDFVDHVDMV EPIDGVVLVD PEYLKGRKMF VMLTCAFRYG HDDLDVIGLT FRKDLYVQVQ QVVPAESSSP RGSLTVLQER LLHKLGDNAY PFTLQMVVNL PCSVTLQPGP DDTGKACGVD FEVKSFCAEN LEEKVSKRDS VRLVIRKIQF APLEPGPGPW ARLCRRFLLS AQPLLLQAWM DKEVNYHGQP ISVNVSINNS TNKVIKKIKI SVDQITDVVL YSLDKYTKTV FVQEFTETIA ANSTFSKSFA VTPLLADNCH KQGLALDGKL KQGDTNLASS TILRPGVDKE LLGILVSYKV RVNLMVSCEG ILGDLTASEV GVELPLILMH PKPSNEAASS EDIVIEEFAQ QEPSGESQEA LAAEGNEGS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in an as yet undefined retina-specific signal transduction. Could bind to photoactivated-phosphorylated red/green opsins.
Gene References into Functions
  1. Inositol hexakisphosphate (IP6) binds to the arrestin-3 phosphate sensor, and is stabilized by trimerization. Analysis of the trimerization surface, which is also the receptor-binding surface, suggests a feature called the finger loop as a key region of the activation sensor. PMID: 29127291
  2. Several mutations in the two parts of the central "crest" of the arrestin molecule, middle-loop and C-loop, enhanced or reduced arrestin-3 interactions with several GPCRs in receptor subtype and functional state-specific manner. PMID: 28461104
  3. K2A mutations in arrestin-1, -2, and -3 significantly reduced their binding to active phosphorhodopsin. PMID: 22275358
  4. multiple residues on the non-receptor-binding side of arrestin-3 are crucial for JNK3 activation PMID: 21715332
  5. Both nonvisual arrestin-2 and arrestin-3 are shown to directly bind Jun kinase (JNK)3alpha2 and its upstream activator Map kinase kinase (MKK)4; the affinity of arrestin-3 for these kinases is higher than that of arrestin-2. PMID: 22047447
  6. the first crystal structure of arrestin-3, solved at 3.0 A resolution. Receptor binding. PMID: 21215759
  7. SUMOylation sites in arrestin-3; arrestin-3 SUMOylation mediates beta(2)AR internalization PMID: 21118812
  8. The nature of the changes in arrestin 3 distribution observed upon activation of adenosine receptors correlates with receptor sensitivity to G-protein-coupled receptor kinase-mediated phosphorylation and rapid internalization. PMID: 12475223
  9. microtubule interaction may play a role in keeping p44 arrestin away from rhodopsin in dark-adapted photoreceptors PMID: 15272005

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Subcellular Location
Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.
Protein Families
Arrestin family
Tissue Specificity
Expressed in cone photoreceptors in the retina (at protein level).
Database Links

KEGG: bta:281011

STRING: 9913.ENSBTAP00000017088

UniGene: Bt.494

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