Recombinant Bovine Beta-defensin 4 (DEFB4)

Code CSB-YP338444BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP338444BO
MSDS
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Source E.coli
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Code CSB-EP338444BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP338444BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP338444BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DEFB4
Uniprot No.
Alternative Names
DEFB4; BNBD4Beta-defensin 4; BNBD-4; BNDB-4
Species
Bos taurus (Bovine)
Expression Region
23-63
Target Protein Sequence
QRVRNPQS CRWNMGVCIP FLCRVGMRQI GTCFGPRVPC CRR
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has bactericidal activity. Active against E.coli ML35 and S.aureus 502A.
Gene References into Functions
  1. Bovine DEFB4 mRNA is upregulated by lipopolysaccharide in bovine neutrophils and mammary epithelial cells and by 1,25-dihydroxyvitamin D3 in bovine monocytes and mammary epithelial cells. PMID: 26255277
  2. Recombinant mature bovine neutrophil beta-defensin 4 inhibits mycobacterial growth and controls intracellular survival of mycobacteria. PMID: 25946365
  3. Stimulation of umbilical-vein endothelial cells with S. aureus or LPS induced the expression of bovine neutrophil beta-defensin 4. This expression was regulated by the autocrine production of tumor necrosis factor-alpha. PMID: 23298865
  4. The isolation of polymorphisms of BNBD-4 and milk production and somatic cell count are reported. PMID: 17550357
Subcellular Location
Secreted.
Protein Families
Beta-defensin family
Tissue Specificity
Neutrophilic granules.
Database Links

KEGG: bta:286836

STRING: 9913.ENSBTAP00000055285

UniGene: Bt.146

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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