Recombinant Bovine Cytochrome P450 19A1 (CYP19A1), partial

Code CSB-YP006394BO
MSDS
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Source Yeast
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Code CSB-EP006394BO
MSDS
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Source E.coli
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Code CSB-EP006394BO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006394BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006394BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP19A1
Uniprot No.
Alternative Names
CYP19A1; CYP19Aromatase; EC 1.14.14.14; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase
Species
Bos taurus (Bovine)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Gene References into Functions
  1. in granulosa cells of dominant follicles the concentration of CYP19P1 mRNA was very low compared to CYP19A1 mRNA. CONCLUSIONS: CYP19P1 and CYP19A1 transcripts might interfere in placental cotyledons PMID: 24947985
  2. These data suggest that a major reason why CYP19A1 is not expressed in luteinized cells (and the corpus luteum) of ruminants may be the stimulatory effect of FOXL2, which does not appear to be the case in the human and rat. PMID: 24556528
  3. This study evaluated the relationships among aromatase, IGF-1, IGF2R, and FSH levels expressed in ovarian follicles of cattle selected for twin pregnancies. PMID: 22266997
  4. Data indicated de novo DNA methylation fter the luteinizing hormone-induced down-regulation of CYP19A1 expression, suggesting that DNA methylation might play a role for permanent silencing of previously down-regulated genes. PMID: 22531467
  5. P450(AROM) is localized in the cytoplasm and only seldom present in the fine extensions of the cells in frontal cortex. PMID: 21397601
  6. Granulosa and theca of well-characterized large bovine follicles were isolated before and after the LH surge. CYP19A1, HSD3B1, and CYP17A1 transcripts were quantified by real-time PCR (qPCR) and the chromatin condensation was determined. PMID: 21069797
  7. DNA methylation may have a role in the permanent shutdown of promoter 2-directed cytochrome P450 19A1(CYP19A1) expression in large (granulosa derived) lutein cells PMID: 19794152
  8. fetal ovary of cattle has the steroidogenic enzyme aromatase to convert androgens to estradiol-17beta, and estrogen receptors alpha and beta to facilitate an estrogen response within the fetal ovary PMID: 19299095
  9. granulosa cell clustering is accompanied by marked increases in FSHr, IGF-1r, and p450 arom expression, and precedes induction and subsequent peak E2 production PMID: 12782408
  10. demonstrates that cattle and sheep use different promoters to direct aromatase(Cyp19) expression during pregnancy PMID: 15219930
  11. Promoter-2-derived high-level expression of Cyp19 might be controlled by cell-type-specific DNA methylation. PMID: 15767046
  12. CYP19 is regulated by multiple pathways in granulosa cells PMID: 16641147
  13. second quarter of the gestation is the developmental stage which represents a critical period for hypothalamic differentiation in bovine ontogenesis. PMID: 18158096
  14. Serum free culture conditions significantly enhanced Cyp17 and Csh1 but not Hsd3b expression in trophoblast. PMID: 18455233
  15. CYP19 promoters are differentially methylated in ovine and bovine placental tissues of foetal and maternal origin. PMID: 18523913
  16. Administration of CART in vivo into follicles at the early dominance stage reduced follicular fluid estradiol concentrations and granulosa cell CYP19A1 mRNA. PMID: 19439726
  17. The CYP19/PvuII allele frequencies were as follows: A -0.91 and B -0.09. The highest average heterozygosity rate was found in herd A (0.2108). PMID: 19705744

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
Protein Families
Cytochrome P450 family
Database Links
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