Recombinant Bovine DNA polymerase delta subunit 3(POLD3)

Product Type Recombinant Protein
Uniprot No. P84798
Species Bos taurus (Bovine)
Gene Names POLD3
Protein Names Recommended name: DNA polymerase delta subunit 3 Alternative name(s): DNA polymerase delta subunit p66 p68
Storage Store at -20°C, for extended storage, conserve at -20°C or -80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
HGNC 20932
RGD 1312027
MGI 1915217
Function As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (By similarity). Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (By similarity).
Subcellular Location Cytoplasm, Nucleus
Database Links

STRING: 9913.ENSBTAP00000042403

UniGene: Bt.15674

Pathway DNA repair pathway

Product Details for Different Expression Systems

Product Name Recombinant Bovine DNA polymerase delta subunit 3(POLD3),Yeast
Code CSB-YP018305BO
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence ADQFYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYDYVERKRK ENSGAQLHVT YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASVHV YSIQKAMLKD SGPLFNTDYD ILKSNLQNCS KFSAIQCAAA VPRAPAESSS SEKLEQSDPP VSPEMQASDE LTTNGHGPPV PKQSSQQPKG IMGMFASKAA SKAQDANKET KTEAKEVMNS EKNNENLLLS GYWESMIVLF QAQCINKLKV NLDSEQEVKE EKKVEQPPLS VTEPKLAAPV DLKKSSKKAE PVRMQQKEKK RRKQMELSDD ETKETENMKK KRRRIKLPES DSSEDEVIPD SPGAYEAESP SPPPPSPSPE PVLKTEPEPP PVKGSDGENK RKRKRVLKSK TFTDEEGCMV TEKVYESESC TDSEEELKMK TSSVHRPPAM AVKKEPKEER KGPKKGTAAM GKANRQVAIT GFFQRK
Expression Region 2-466
Protein Description Full Length of Mature Protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Bovine DNA polymerase delta subunit 3(POLD3),E.coli
Code CSB-EP018305BO
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence ADQFYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYDYVERKRK ENSGAQLHVT YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASVHV YSIQKAMLKD SGPLFNTDYD ILKSNLQNCS KFSAIQCAAA VPRAPAESSS SEKLEQSDPP VSPEMQASDE LTTNGHGPPV PKQSSQQPKG IMGMFASKAA SKAQDANKET KTEAKEVMNS EKNNENLLLS GYWESMIVLF QAQCINKLKV NLDSEQEVKE EKKVEQPPLS VTEPKLAAPV DLKKSSKKAE PVRMQQKEKK RRKQMELSDD ETKETENMKK KRRRIKLPES DSSEDEVIPD SPGAYEAESP SPPPPSPSPE PVLKTEPEPP PVKGSDGENK RKRKRVLKSK TFTDEEGCMV TEKVYESESC TDSEEELKMK TSSVHRPPAM AVKKEPKEER KGPKKGTAAM GKANRQVAIT GFFQRK
Expression Region 2-466
Protein Description Full Length of Mature Protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Bovine DNA polymerase delta subunit 3(POLD3),Baculovirus
Code CSB-BP018305BO
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence ADQFYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYDYVERKRK ENSGAQLHVT YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASVHV YSIQKAMLKD SGPLFNTDYD ILKSNLQNCS KFSAIQCAAA VPRAPAESSS SEKLEQSDPP VSPEMQASDE LTTNGHGPPV PKQSSQQPKG IMGMFASKAA SKAQDANKET KTEAKEVMNS EKNNENLLLS GYWESMIVLF QAQCINKLKV NLDSEQEVKE EKKVEQPPLS VTEPKLAAPV DLKKSSKKAE PVRMQQKEKK RRKQMELSDD ETKETENMKK KRRRIKLPES DSSEDEVIPD SPGAYEAESP SPPPPSPSPE PVLKTEPEPP PVKGSDGENK RKRKRVLKSK TFTDEEGCMV TEKVYESESC TDSEEELKMK TSSVHRPPAM AVKKEPKEER KGPKKGTAAM GKANRQVAIT GFFQRK
Expression Region 2-466
Protein Description Full Length of Mature Protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Bovine DNA polymerase delta subunit 3(POLD3),Mammalian cell
Code CSB-MP018305BO
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence ADQFYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYDYVERKRK ENSGAQLHVT YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASVHV YSIQKAMLKD SGPLFNTDYD ILKSNLQNCS KFSAIQCAAA VPRAPAESSS SEKLEQSDPP VSPEMQASDE LTTNGHGPPV PKQSSQQPKG IMGMFASKAA SKAQDANKET KTEAKEVMNS EKNNENLLLS GYWESMIVLF QAQCINKLKV NLDSEQEVKE EKKVEQPPLS VTEPKLAAPV DLKKSSKKAE PVRMQQKEKK RRKQMELSDD ETKETENMKK KRRRIKLPES DSSEDEVIPD SPGAYEAESP SPPPPSPSPE PVLKTEPEPP PVKGSDGENK RKRKRVLKSK TFTDEEGCMV TEKVYESESC TDSEEELKMK TSSVHRPPAM AVKKEPKEER KGPKKGTAAM GKANRQVAIT GFFQRK
Expression Region 2-466
Protein Description Full Length of Mature Protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.