Recombinant Bovine Epididymal secretory protein E1(NPC2)

Code CSB-YP015976BO
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Source Yeast
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Code CSB-EP015976BO
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Source E.coli
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Code CSB-EP015976BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015976BO
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Source Baculovirus
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Code CSB-MP015976BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NPC2
Uniprot No. P79345
Alternative Names NPC2; NPC intracellular cholesterol transporter 2; EPV20; Epididymal secretory protein E1; Niemann Pick type C2 protein homolog
Species Bos taurus (Bovine)
Expression Region 20-149
Target Protein Sequence E PVKFKDCGSW VGVIKEVNVS PCPTQPCKLH RGQSYSVNVT FTSNTQSQSS KAVVHGIVMG IPVPFPIPES DGCKSGIRCP IEKDKTYNYV NKLPVKNEYP SIKVVVEWEL TDDKNQRFFC WQIPIEVEA
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
Gene References into Functions
  1. Data suggest ASM (acid sphingomyelinase) activity is regulated by membrane lipids and facilitates cholesterol transfer by NPC2 (Niemann Pick protein type C2); hydrolysis of sphingomyelin by ASM may be crucial for endosomal lipid degradation/sorting. PMID: 25339683
  2. Data show that the effects of the lipids on cholesterol transfer mediated by NPC2 were similar to their effect on membrane fusion induced by NPC2 and saposin-C. PMID: 20179319
  3. bNPC2 has a loosely packed region penetrating from the surface into the hydrophobic core that forms adjacent small cavities and represents an incipient cholesterol-binding site PMID: 12591954
  4. analysis of sterol binding by NPC2, a lysosomal protein deficient in Niemann-Pick type C2 disease PMID: 17573352
  5. The seminal plasma protein, Niemann-Pick C2 protein, is involved in cholesterol and GM1 depletion within detergent-resistant membrane, then leading to membrane redistribution of P25b that occurs in a very rapid and capacitation-independent manner. PMID: 18235103
  6. NPC2 plays an important role in endo/lysosomal cholesterol trafficking by markedly accelerating the rates of cholesterol transport. Rates of sterol transfer from and between membranes were increased by as much as 2 orders of magnitude by NPC2. PMID: 18823126

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Subcellular Location Secreted, Endoplasmic reticulum, Lysosome
Protein Families NPC2 family
Tissue Specificity Expressed in kidney, spleen, liver and mammary gland, but not in testis.
Database Links

KEGG: bta:280815

STRING: 9913.ENSBTAP00000029271

UniGene: Bt.23268

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