Recombinant Cat Malate dehydrogenase, cytoplasmic (MDH1)

Code CSB-YP801782CA
MSDS
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Source Yeast
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Code CSB-EP801782CA
MSDS
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Source E.coli
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Code CSB-EP801782CA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP801782CA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP801782CA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MDH1
Uniprot No.
Alternative Names
MDH1Malate dehydrogenase; cytoplasmic; EC 1.1.1.37; Cytosolic malate dehydrogenase
Species
Felis catus (Cat) (Felis silvestris catus)
Expression Region
2-334
Target Protein Sequence
SEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRRDGMERKD LLKANVKIFK CQGAALEKYA KKSVKVIVVG NPANTNCLTA CKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTSDDVKN VIIWGNHSST QYPDVSHAKV KLHGKEVGVY DALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG IISDGNPYGV PDDLLYSFPV TIKNKTWKVV EGLTINDFSR EKMDLTAKEL AEEKETAFEF LSSA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
Gene References into Functions
  1. Data indicate that both cytosolic and mitochondrial malate dehydrogenases (MDH1 and MDH2) mRNAs were ubiquitously expressed, but these expression levels varied in a tissue-specific manner. PMID: 25177965
Subcellular Location
Cytoplasm.
Protein Families
LDH/MDH superfamily, MDH type 2 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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