Recombinant Caulobacter crescentus Chromosomal replication initiator protein DnaA (dnaA)

Code CSB-YP316417DQO
MSDS
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Source Yeast
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Code CSB-EP316417DQO
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Source E.coli
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Code CSB-EP316417DQO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP316417DQO
MSDS
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Source Baculovirus
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Code CSB-MP316417DQO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dnaA
Uniprot No.
Alternative Names
dnaA; CC_0008Chromosomal replication initiator protein DnaA
Species
Caulobacter crescentus (strain ATCC 19089 / CB15)
Expression Region
1-490
Target Protein Sequence
MTMKGGVASQ DFSAAIATAC EPAANVWSKV CVALKRELGD AAFGSWIAPA MLREAATGDV VLVTSTGIAR DWIRRSAWRR IGELWAAHDA TGRRIDLKSR LEFEAAAGAY VEATPKAVAA EPIEIVLPVS TDAPTVVAPS AKSPRTQGLQ ERFTFETFVP GPANEFAHAV ARRIANWADG HFNPVLFHGP YGFGKTHLLN ALAWEAMRNA PEKRVVYLTA ERFLSTFVRA VMDRQTAAFK EELRAADLLI IDDVHFIAGK QSTQEELFHT LTALVGEGGR VVFSADRPPS AMTEMDAHLR SHLSAGLVCG LEPADRNLRL GILERKIQTL GAAHGFEPSI RPEVMQFLAD RFTDSVRELE GALNTLSARA GEGLSRMTLD EVQAILRPHL RSGEKRITID DIQKATAEHY GMKQADLLSE RRNRAVARPR QAAMWLAKQL TTRSLPDIGR RFGGRDHTTV LHAVRRIEAL RAEDSALSHD LETLTRKLRG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
Gene References into Functions
  1. Study presented evidence that a sophisticated regulatory network involving the DnaK/Hsp70 chaperone and the protease Lon controls DnaA stability in C. crescentus. PMID: 23911325
  2. Upon entry into stationary phase DnaA protein is completely removed PMID: 15686567
  3. coordinates DNA replication initiation with cell cycle progression by acting as a global transcription factor. PMID: 16313620
  4. DnaA couples DNA replication initiation with the expression of the two oscillating regulators GcrA and CtrA and that the DnaA/GcrA/CtrA regulatory cascade drives the forward progression of the Caulobacter cell cycle. PMID: 16395331
Subcellular Location
Cytoplasm.
Protein Families
DnaA family
Database Links

KEGG: ccr:CC_0008

STRING: 190650.CC_0008

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