Recombinant Caulobacter crescentus Transcriptional regulatory protein flbD (flbD)

Code CSB-YP325539DQO
MSDS
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Source Yeast
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Code CSB-EP325539DQO
MSDS
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Source E.coli
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Code CSB-EP325539DQO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP325539DQO
MSDS
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Source Baculovirus
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Code CSB-MP325539DQO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
flbD
Uniprot No.
Alternative Names
flbD; ftrC; CC_0909Transcriptional regulatory protein FlbD
Species
Caulobacter crescentus (strain ATCC 19089 / CB15)
Expression Region
1-455
Target Protein Sequence
MRLLVVGKLN GQLSVAVKMA MNAGAKVSHV ETTEQATNAL RAGQGADLLM VDYVLDIAGL IAANEAERMR VPVVACGVDA DPMRAANAIK AGAKEFIPLP PDAELIAAVL AAVTDDEKPM VVRDPAMEQV IKLADQVAPS EASILITGES GSGKEVMARY VHGKSRRAKA PFISVNCAAI PENLLESELF GHEKGAFTGA MARRIGKFEE ADGGTLLLDE ISEMDVRLQA KLLRAIQERE IDRVGGSKPV KVNIRILATS NRDLAQAVKD GTFREDLLYR LNVVNLRLPP LRERPADVIS LCEFFVKKYS AANGIEEKPI SAEAKRRLIA HRWPGNVREL ENAMHRAVLL SAGPEIEEFA IRLPDGQPMA PAPDVAVARG AQMAADAASR AFVGSTVAEV EQQLIIDTLE HCLGNRTHAA NILGISIRTL RNKLKEYSDA GVQVPPPQGG VGAAA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of FlbD in C.crescentus is controlled by an internal cell-cycle clock.
Gene References into Functions
  1. Data show that FliX functions as a negative regulator of FlbD activity in the absence of the class II-encoded flagellar structure. PMID: 15491362
Subcellular Location
Cytoplasm.
Database Links

KEGG: ccr:CC_0909

STRING: 190650.CC_0909

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