Recombinant Chicken Myoblast determination protein 1 homolog (MYOD1)

Code CSB-YP015358CH
MSDS
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Source Yeast
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Code CSB-EP015358CH
MSDS
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Source E.coli
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Code CSB-EP015358CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015358CH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP015358CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYOD1
Uniprot No.
Alternative Names
MYOD1; CMD1; MYOD; Myoblast determination protein 1 homolog; MYOD1 homolog
Species
Gallus gallus (Chicken)
Expression Region
1-298
Target Protein Sequence
MDLLGPMEMT EGSLCSFTAA DDFYDDPCFN TSDMHFFEDL DPRLVHVGGL LKPEEHPHTR APPREPTEEE HVRAPSGHHQ AGRCLLWACK ACKRKTTNAD RRKAATMRER RRLSKVNEAF ETLKRCTSTN PNQRLPKVEI LRNAIRYIES LQALLREQED AYYPVLEHYS GESDASSPRS NCSDGMMEYS GPPCSSRRRN SYDSSYYTES PNDPKHGKSS VVSSLDCLSS IVERISTDNS TCPILPPAEA VAEGSPCSPQ EGGNLSDSGA QIPSPTNCTP LPQESSSSSS SNPIYQVL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Gene References into Functions
  1. Vgl-2 acts downstream of MyoD activation and is associated with the differentiation step in embryonic skeletal myogenesis PMID: 19833199
  2. MyoD expression is regulated by Hoxa-11 and Hoxa-13 in limb muscle precursors PMID: 14706073
  3. These results demonstrate that MyoD-positive epiblast cells recruit pluripotent cells to the skeletal muscle lineage through a mechanism thatinvolves blocking the BMP signaling pathway [MyoD]. PMID: 14981095
  4. NeuroM and MyoD are present in separate subpopulations of cells in the pregastrulating epiblast; epiblast cells with NeuroM are more dependent on exogenous factors to differentiate than those with MyoD PMID: 15661645
  5. MyoD-positive epiblast cells are stasbly committed to the skeletal muscle cell lineage. PMID: 17698608

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Subcellular Location
Nucleus.
Database Links

KEGG: gga:374048

UniGene: Gga.750

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