Greater than 90% as determined by SDS-PAGE.
botBBotulinum neurotoxin type B; BoNT/B; Bontoxilysin-B) [Cleaved into: Botulinum neurotoxin B light chain; LC; EC 18.104.22.168); Botulinum neurotoxin B heavy chain; HC)]
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The production of this Recombinant C. botulinum botB protein started with the botB gene synthesis. And then using recombinant DNA technology, the botB gene was inserted into an expression vector so that we could get the recombinant express plasmid of botB. Transform the plasmid into the cells of Yeast, culture the cells and we could get the desired Recombinant C. botulinum botB protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE.
botB is a gene providing an instruction of making a protein named botulinum neurotoxin type B (BoNT/B) in clostridium botulinum. The protein encoded by this gene is also known as bontoxilysin-B and belongs to peptidase M27 family. This protein can be cleaved into two chains, including botulinum neurotoxin B light chain (LC) and botulinum neurotoxin B heavy chain (HC). It mainly targets motor nerve terminals and block neurotransmitter release, resulting in muscle paralysis.