Recombinant Cysteine protease avirulence protein AvrRpt2 (avrRpt2)

Code CSB-YP764351PWG
MSDS
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Source Yeast
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Code CSB-EP764351PWG
MSDS
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Source E.coli
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Code CSB-EP764351PWG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP764351PWG
MSDS
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Source Baculovirus
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Code CSB-MP764351PWG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
avrRpt2
Uniprot No.
Alternative Names
avrRpt2Cysteine protease avirulence protein AvrRpt2; Avirulence protein AvrRpt2; EC 3.4.22.-
Species
Pseudomonas syringae pv. tomato
Expression Region
72-255
Target Protein Sequence
GWFKKKSSK HETGGSSANA DSSSVASDST EKPLFRLTHV PYVSQGNERM GCWYACARMV GHSVEAGPRL GLPELYEGRE GPAGLQDFSD VERFIHNEGL TRVDLPDNER FTHEELGALL YKHGPIIFGW KTPNDSWHMS VLTGVDKETS SITFHDPRQG PDLAMPLDYF NQRLAWQVPH AMLYR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Effector protein involved in gene-for-gene resistance in plants expressing RPS2. Its thiol protease activity is required for the degradation of plant cell RIN4 and consequent activation of RPS2 during bacterial infection. The activation of RPS2 is sufficient for the induction of hypersensitive response (HR) and plant resistance. Cleavage of RIN4 by AvrRpt2 also interferes with RPM1-mediated resistance activated by either AvrRpm1 or AvrB. Contributes to virulence in plants lacking the resistance protein RPS2 promoting pathogen growth and disease symptoms. Inhibits PAMP (pathogen-associated molecular patterns)-induced signaling compromising the host's basal defense system. Blocks plant callose deposition, flg22 (a peptide corresponding to the most conserved domain of flagellin) induced accumulation of PR-1, PR-2 and PR-5 and activation of GST6 transcription. The mechanism of virulence is unknown, but this activity is independent of ethylene and salicylic acid response pathways and independent of RIN4 disappearance.
Subcellular Location
Secreted. Host cell membrane. Note=Secreted via type III secretion system (TTSS) (PubMed:14526114). Localized to the plant cell membrane (PubMed:14526114).
Protein Families
Peptidase C70 family
Database Links

KEGG: ag:CAA79815

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