Recombinant Drosophila melanogaster Guanine nucleotide-binding protein subunit gamma-1 (Ggamma1)

Code CSB-YP334358DLU
MSDS
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Source Yeast
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Code CSB-EP334358DLU
MSDS
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Source E.coli
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Code CSB-EP334358DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334358DLU
MSDS
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Source Baculovirus
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Code CSB-MP334358DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ggamma1
Uniprot No.
Alternative Names
Ggamma1; G1; CG8261; Guanine nucleotide-binding protein subunit gamma-1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-67
Target Protein Sequence
MDVMSSSLQQ QRVVVEQLRR EAAIDRQTIS ESCAKMMKYI TEHEQEDYLL TGFTSQKVNP FREKSSC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Gene References into Functions
  1. Data identify a mutant in the Ggamma1 subunit of heterotrimeric G protein, which produces Ggamma1 lacking its membrane anchor site and exhibits phenotypes identical to those of Gbeta13F, including abnormal spindle asymmetry and spindle orientation. PMID: 14981094
  2. G-protein gamma subunit 1 participates in the signal transduction of sugar taste reception PMID: 16121192
  3. findings reveal a cardial cell-autonomous requirement of Ggamma1 geranylgeranylation for heart formation and suggest the involvement of the mevalonate pathway in congenital heart disease PMID: 16857902
  4. findings show that Ggamma1 is a component of the Hedgehog signaling pathway & is required for the efficient release of the Hh ligand from hh expressing cells; also Ggamma1 is required for germ cell migration PMID: 19132091
Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side.
Protein Families
G protein gamma family
Tissue Specificity
Predominantly expressed in the central nervous system.
Database Links

KEGG: dme:Dmel_CG8261

STRING: 7227.FBpp0087728

UniGene: Dm.2812

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