Product Details

Purity

>85% (SDS-PAGE)

Target Names

Mef2

Uniprot No.

P40791

Alternative Names

Mef2; CG1429Myocyte-specific enhancer factor 2; D-mef2; MADS domain transcription factor

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcription factor that could be a key player in early mesoderm differentiation and may be required for subsequent cell fate specifications within the somatic and visceral/heart mesodermal layers. Essential for myoblast fusion and consequently muscle formation in adults. During embryonic and pupal development, binds to the enhancer of the myoblast fusion gene sing and activates its transcription.

Gene References into Functions

Overexpression of Tinman and Pannier resulted in 20% of embryos with ectopic Hand and Sur expression. By adding MEF2 alongside Tinman and Pannier, an expansion in expression of Hand and Sur was observed. PMID: 26225919
Our results show that Mef2 and possibly Sd regulate a Dl enhancer specifically expressed in the developing indirect flight muscles and that Mef2 is required for Dl expression in developing indirect flight muscles. PMID: 25247309
critical switch between anabolic and immune function in the adult Drosophila fat body [review] PMID: 25483252
MEF2 directly controls fusion at multiple stages of the life cycle PMID: 25797154
The loss of phosphorylated MEF2 contributes to loss of anabolic enzyme expression in Gram-negative bacterial infection. MEF2 is thus a critical transcriptional switch in the adult fat body between metabolism and immunity. PMID: 24075010
Mutation of MEF2 sites severely impaired both spontaneous and induced expression of not only BZLF1, but also BRLF1 in comparison to wild-type or revertant virus cases. PMID: 23843637
Mef2 transmits circadian clock information in neuronal plasticity, which contributes to locomotor activity rhythms. PMID: 23889933
The negative feedback circuit between miR-92b and Mef2 efficiently maintains the stable expression of both components that is required for homeostasis during Drosophila muscle development. PMID: 22899845
transcription factor Mef2 has multiple roles in adult Drosophila musculature formation PMID: 22357930
MEF2 remains an essential factor, participating in control of myoblast fusion, and myofibrillogenesis in developing myotubes. PMID: 22008792
Lmd is a tissue-specific modulator of Mef2 activity, acting as both a transcriptional activator and repressor. PMID: 20617173
a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms PMID: 20427646
Temperature sensitivity for mutations affecting the Drosophila muscle regulator Myocyte enhancer factor-2. PMID: 19564485
Drosophila MEF2 is a direct regulator of Actin57B transcription in cardiac, skeletal, and visceral muscle lineages. PMID: 11744367
Adult myogenesis in Drosophila can proceed independently of Mef2. PMID: 15956678
expression of the muscle differentiation gene Myocyte enhancer factor-2 (Mef2) is normally delayed in twist-expressing adult myoblasts until the end of the third larval instar and it is an ecdysone-dependent event PMID: 16325168
the use of ChEST in Drosophila to identify several previously unknown targets of myocyte enhancer factor 2 (MEF2), a key regulator of myogenic differentiation PMID: 16339902
mef2 directly regulates target genes at all stages of muscle development. PMID: 16740481
suggests a route by which mef2 can orchestrate the muscle differentiation program and contribute to the stringent regulation of gene expression during myogenesis PMID: 18198273

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Subcellular Location

Nucleus.

Protein Families

MEF2 family

Tissue Specificity

Expressed in all presumptive mesoderm prior to the splitting process that generates the somatic and visceral/ heart mesoderm. After the subdivision, it is found in both the somatic and the visceral/heart mesoderm.

Database Links

KEGG: dme:Dmel_CG1429

UniGene: Dm.7054

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Recombinant Drosophila melanogaster Myocyte-specific enhancer factor 2 (Mef2), partial

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