Recombinant Echis carinatus Zinc metalloproteinase-disintegrin-like ecarin

Code CSB-YP845861EAH
MSDS
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Source Yeast
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Code CSB-EP845861EAH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP845861EAH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP845861EAH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
N/A
Uniprot No.
Alternative Names
Zinc metalloproteinase-disintegrin-like ecarin; EC 3.4.24.-; Snake venom metalloproteinase; SVMP
Species
Echis carinatus (Saw-scaled viper)
Expression Region
191-616
Target Protein Sequence
VPPHERKFEK KFIELVVVVD HSMVTKYNND STAIRTWIYE MLNTVNEIYL PFNIRVALVG LEFWCNGDLI NVTSTADDTL HSFGEWRASD LLNRKRHDHA QLLTNVTLDH STLGITFVYG MCKSDRSVEL ILDYSNITFN MAYIIAHEMG HSLGMLHDTK FCTCGAKPCI MFGKESIPPP KEFSSCSYDQ YNKYLLKYNP KCILDPPLRK DIASPAVCGN EIWEEGEECD CGSPADCRNP CCDAATCKLK PGAECGNGEC CDKCKIRKAG TECRPARDDC DVAEHCTGQS AECPRNEFQR NGQPCLNNSG YCYNGDCPIM LNQCIALFSP SATVAQDSCF QRNLQGSYYG YCTKEIGYYG KRFPCAPQDV KCGRLYCLDN SFKKNMRCKN DYSYADENKG IVEPGTKCED GKVCINRKCV DVNTAY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Snake venom zinc metalloproteinase that catalyzes the conversion of prothrombin (F2) to alpha-thrombin through formation of a thrombin intermediate. Has a low Km for prothrombin and a high kcat. Cleaves the 320-Arg-Ile-321 bond in prothrombin and produces meizothrombin which is ultimately converted to alpha-thrombin by autolysis.
Subcellular Location
Secreted.
Protein Families
Venom metalloproteinase (M12B) family, P-III subfamily, P-IIIa sub-subfamily
Tissue Specificity
Expressed by the venom gland.
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