Recombinant Gloydius brevicaudus Zinc metalloproteinase/disintegrin

Code CSB-YP835467GGO
MSDS
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Source Yeast
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Code CSB-EP835467GGO
MSDS
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Source E.coli
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Code CSB-EP835467GGO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835467GGO
MSDS
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Source Baculovirus
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Code CSB-MP835467GGO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
Zinc metalloproteinase/disintegrin [Cleaved into: Snake venom metalloproteinase brevilysin L4; SVMP; Snake venom metalloproteinase hxl-1; EC 3.4.24.-); Disintegrin salmosin-1; Platelet aggregation inhibitor); Disintegrin salmosin-1 minor component; Disintegrin brevicaudin-2b)]; Fragment
Species
Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus)
Expression Region
27-228
Target Protein Sequence
TPAH QRYIELVIVA DHGMFTKYNG DSDKIREWVR QMVNTVDEIY SYMYIDVALA GLEIWSNEDL INVQPAAPHT LDSFGKWRER DLLHRIHHDN AMLLTAIDFD GPTIGLAYVG TMCKPKGSTG VVQDHSTINL RVAVTMAHEI GHNLGIHHDT GSCSCGGYSC IMSPVISHEP SKYFSDCSYT QCWDFIMNQK PQCILNKP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
metalloproteinase that impairs hemostasis in the envenomed animal.; inhibits GPIIb/GPIIIa (ITGA2B/ITGB3) binding to immobilized fibrinogen with an IC(50) of 2.2 nM and ADP-induced platelet aggregation with an IC(50) of 131 nM, respectively. Inhibits angiogenesis. By binding to vitronectin receptor (alpha-V/beta-3 (ITGAV/ITGB3)), also induces apoptosis of endothelial cells by blocking their attachment to extracellular matrix proteins.; inhibits platelet aggregation induced by ADP (IC(50) is 30 nM), collagen (IC(50) is 500 nM), thrombin and epinephrin (IC(50) is 160 nM).
Subcellular Location
Secreted.
Protein Families
Venom metalloproteinase (M12B) family, P-II subfamily, P-IIa sub-subfamily
Tissue Specificity
Expressed by the venom gland.
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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