Recombinant Human BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3 (KCTD10)

Code CSB-YP872486HU
MSDS
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Source Yeast
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Code CSB-EP872486HU
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Source E.coli
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Code CSB-EP872486HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872486HU
MSDS
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Source Baculovirus
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Code CSB-MP872486HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KCTD10
Uniprot No.
Alternative Names
KCTD10; ULR061; MSTP028BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3; hBACURD3; BTB/POZ domain-containing protein KCTD10; Potassium channel tetramerization domain-containing protein 10
Species
Homo sapiens (Human)
Expression Region
1-313
Target Protein Sequence
MEEMSGESVV SSAVPAAATR TTSFKGTSPS SKYVKLNVGG ALYYTTMQTL TKQDTMLKAM FSGRMEVLTD SEGWILIDRC GKHFGTILNY LRDGAVPLPE SRREIEELLA EAKYYLVQGL VEECQAALQN KDTYEPFCKV PVITSSKEEQ KLIATSNKPA VKLLYNRSNN KYSYTSNSDD NMLKNIELFD KLSLRFNGRV LFIKDVIGDE ICCWSFYGQG RKIAEVCCTS IVYATEKKQT KVEFPEARIY EETLNILLYE AQDGRGPDNA LLEATGGAAG RSHHLDEDEE RERIERVRRI HIKRPDDRAH LHQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(BACURD3) E3 ubiquitin ligase complex mediates the ubiquitination of target proteins, leading to their degradation by the proteasome.
Gene References into Functions
  1. These findings suggest that rs11066782 in KCTD10, rs11613718 in KCTD10 and rs11067233 in MMAB may contribute to the susceptibility of coronary heart disease by altering plasma HDL-C levels in Han Chinese. PMID: 27716295
  2. The gastrointestinal stromal tumor-specific transcription factor ETV1 may have no prognostic potential, whereas its downstream gene KCTD10 is associated with a favorable prognosis. PMID: 23977394
  3. KCTD10 inhibited the transcriptional activities of nuclear factor kappa B (NF-kappaB) and activating protein-1 reporters PMID: 22810651
  4. KCTD10 may be associated with DNA synthesis and cell proliferation PMID: 19125419
  5. binding of SP1 to the proximal promoter region stimulated the promoter activity and endogenous KCTD10 expression, whereas binding of AP-2alpha to this region showed opposite effects. PMID: 19154347
  6. For the SNPs KCTD10_i5642G-->C and MVK_S52NG-->A, homozygotes for the major alleles (G) had lower HDL-cholesterol concentrations than did carriers of the minor alleles (P = 0.005 and P = 0.019, respectively). PMID: 19605566

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Subcellular Location
Nucleus.
Protein Families
BACURD family
Database Links

HGNC: 23236

OMIM: 613421

KEGG: hsa:83892

STRING: 9606.ENSP00000228495

UniGene: Hs.524731

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