The expression of recombinant human COL4A1 protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal GST tag sequence to the designated sequence encoding the 30-167aa of the human COL4A1 protein. This N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged recombinant human COL4A1 protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant COL4A1 protein migrated to the band of about 40 kDa molecular weight.
Lately, COL4A1 as a novel oncogene associated with the clinical characteristics of malignancy predicts poor prognosis in glioma. As a basement membrane protein, COL4A1 is commonly expressed in many tissues and cell types. The functions of includes extracellular matrix structural constituent; extracellular matrix structural constituent conferring tensile strength; platelet-derived growth factor binding; protein binding. In recent years, variants of COL4A1 have been observed in a spectrum of brain malformations, such as autosomal dominant porencephaly, schizencephaly, intracranial calcifications, small vessel disease causing strokes and cerebral hemorrhages, and hereditary angiopathy with nephropathy, aneurysms, and muscle cramps (HANAC syndrome). The COL4A1-VEGFD fusion leads to production of mature VEGFD after proteolytic processing, which may act as an autocrine growth factor for tumour cells. Besides, many researches pointed that COL4A1 might be associated various diseases conditions like angiopathy, hereditary, nephropathy, aneurysms, and muscle cramps; leukoencephalopathy.
||Greater than 90% as determined by SDS-PAGE.
||Arresten; BSVD; CO4A1_HUMAN; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Of Basement Membrane Alpha 1 Chain; Collagen Of Basement Membrane Alpha 2 Chain; Collagen Type IV Alpha 1; collagen type IV alpha 1 chain; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RATOR
||Homo sapiens (Human)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.