Recombinant Human Cytochrome P450 27C1 (CYP27C1)

Code CSB-YP675696HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP675696HU
MSDS
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Source E.coli
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Code CSB-EP675696HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP675696HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP675696HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP27C1
Uniprot No.
Alternative Names
C27C1_HUMAN; CYP27C1; Cytochrome P450 27C1; Cytochrome P450 family 27 subfamily C polypeptide 1; Cytochrome P450 family 27 subfamily C polypeptide 13; FLJ16008
Species
Homo sapiens (Human)
Expression Region
1-372
Target Protein Sequence
MRSVLRQRIL KPKDVAIYSG EVNQVIADLI KRIYLLRSQA EDGETVTNVN DLFFKYSMEG VATILYESRL GCLENSIPQL TVEYIEALEL MFSMFKTSMY AGAIPRWLRP FIPKPWREFC RSWDGLFKFS QIHVDNKLRD IQYQMDRGRR VSGGLLTYLF LSQALTLQEI YANVTEMLLA GVDTTSFTLS WTVYLLARHP EVQQTVYREI VKNLGERHVP TAADVPKVPL VRALLKETLR LFPVLPGNGR VTQEDLVIGG YLIPKGTQLA LCHYATSYQD ENFPRAKEFR PERWLRKGDL DRVDNFGSIP FGHGVRSCIG RRIAELEIHL VVIQLLQHFE IKTSSQTNAV HAKTHGLLTP GGPIHVRFVN RK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
A cytochrome P450 monooxygenase that catalyzes the 3,4 desaturation of all-trans-retinol (also called vitamin A1) to all-trans-3,4-didehydroretinol (also called vitamin A2) in the skin. Desaturates with lower efficiency all-trans retinal and all-trans retinoic acid. Forms minor amounts of 3-hydroxy and 4-hydroxy all-trans-retinol derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Gene References into Functions
  1. Data suggest that the most likely catalytic mechanism for conversion of all-trans-retinol to 3,4-dehydroretinol by CYP27C1 begins with abstraction of a hydrogen atom from C-4 (or possibly C-3) initiating desaturation pathway, followed by sequential abstraction of a hydrogen atom or proton-coupled electron transfer. CYP27C1 appears to be localized to skin. PMID: 28701464
Subcellular Location
[Isoform 2]: Mitochondrion membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Widely expressed, with highest levels in the liver, kidney and pancreas.; [Isoform 2]: Expressed in the skin (at protein level).
Database Links

HGNC: 33480

KEGG: hsa:339761

STRING: 9606.ENSP00000334128

UniGene: Hs.407639

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