Recombinant Human Dual specificity protein phosphatase 1 (DUSP1)

Code CSB-YP007238HU
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Source Yeast
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Code CSB-EP007238HU
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Source E.coli
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Code CSB-EP007238HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007238HU
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Source Baculovirus
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Code CSB-MP007238HU
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Protein Length
Full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Gene References into Functions
  1. The molecular structure of DUSP1 crystallized as MBP fusion protein has been reported. PMID: 30198887
  2. High DUSP1 expression is associated with apatinib resistance by activating the MAPK pathway in gastric cancer. PMID: 29956792
  3. SIN attenuated LPS-induced inflammatory injury by regulation of miR-101, MKP-1 and JNK pathway. PMID: 29501764
  4. miR-202-3p is upregulated in type 1 gastric neuroendocrine neoplasms (g-NENs) lesions and might play important roles in the pathogenesis of type 1 g-NENs by targeting DUSP1. PMID: 29434446
  5. Downregulation of the expression of DUSP1 or protein phosphatase 1 led to a decline in the beta2adrenergic receptormediated dephosphorylation of ERK1/2. PMID: 29257221
  6. the expression of DUSP1 was lower in OA FLSs than in normal FLSs, and DUSP1 may inhibit the expression of OA-associated mediators, MMP-13 and COX-2, by suppressing the activation of p38 MAPK and JNK pathways in OA FLSs. PMID: 28983624
  7. Curcumin suppressed CXCL5 expression by direct inhibition of IKKbeta phosphorylation, and inhibition of p38 MAPK via induction of negative regulator MKP-1. PMID: 27538525
  8. MKP-1 is a redox-regulated master controller of monocyte function and macrophage phenotype. (Review) PMID: 28330703
  9. We propose a signaling cascade involving ARID1A, GADD45B and DUSP1 as mediators of the romidepsin effects in GCC cells. PMID: 27572311
  10. this work indicates that suppression of JNK1/2 activity by MKP-1 maintains PARP-1 levels and suggests that MKP-1-mediated cisplatin resistance can be bypassed by PARP-1 inhibition. PMID: 28650468
  11. Mean morning and evening DUSP1 mRNA levels showed significant increase during Ramadan compared to Shabaan, however, its diurnal rhythm was maintained. Morning IL-1alpha mRNA expression remained significantly higher than in the evening during Ramadan, but was markedly decreased compared to Shabaan. PMID: 28384165
  12. Methylation-mediated silencing of the DUSP-1 promoter does not appear to be associated with reduced expression, indicating the involvement of other factors in specific suppression of DUSP-1 in diabetes-associated cardiac hypertrophy. PMID: 28413926
  13. Increased MAP2K6, MAP4K3, and DUSP1 gene expressions in post-chemotherapy samples indicate a poor clinical outcome in osteosarcoma patients. PMID: 28112450
  14. The aim of this review is to shed light on the role of four different phosphatases (PTEN, PP2A, CDC25 and DUSP1) in five different solid tumors (breast cancer, lung cancer, pancreatic cancer, prostate cancer and ovarian cancer), in order to better understand the most frequent and aggressive primary cancer of the central nervous system, glioblastoma. PMID: 28801478
  15. Dual-specific phosphatase (DUSP1) was found to inhibit gallbladder cancer (GBC) cell proliferation, migration and invasion. PMID: 28129656
  16. The results presented here emphasize the importance of MKP-1 as a mediator of therapeutic effects of glucocorticoids in inflammatory lung diseases as well as its potential as a novel anti-inflammatory drug target. PMID: 28299397
  17. Our results emphasize the importance of MKP-1 as a potential predictive biomarker for a subset of breast cancer patients with worse outcome and less susceptibility to treatment. PMID: 27599524
  18. Collectively, these data indicated that DUSP1 may induce the resistance against paclitaxel through the p38 MAPK-mediated overexpression of p-glycoprotein in human ovarian cancer cells. PMID: 27422607
  19. silencing of IL1B plus dexamethasone-induced DUSP1 significantly reduced IRF1 expression. IL1B-induced expression of CXCL10 was largely insensitive to dexamethasone, whereas other DUSP1-enhanced, IRF1-dependent mRNAs showed various degrees of repression. PMID: 27551049
  20. dexamethasone-induced DUSP1 acts via p38 MAPK to switch on the mRNA destabilizing function of protein-tristetraprolin to repress pro-inflammatory cytokine secretion from ASM cells PMID: 26825339
  21. LPS tolerance interferes with TLR4 signaling by inhibiting Lyn and c-Src phosphorylation and their recruitment to TLR4, while increasing the phosphatase activity and expression of PP2A, PTPN22, PTP1B and MKP1. PMID: 26457672
  22. This study shows that RIG-I activation results in MKP-1-mediated inhibition of cell proliferation in melanoma cells via controlling the p38-HSP27, c-Jun and rpS6 pathways PMID: 26829212
  23. In E1a expressing non-small cell lung carcinoma cells, upregulation of MKP1 and the subsequent p38MAPK inhibition is required for the induction of chemosensitivity to cisplatin. PMID: 26689986
  24. The heparin effects on TNFalpha-induced stress fiber formation and Map kinase signaling depend on increased DUSP1 expression. PMID: 26769965
  25. evidence of association between depressive symptom severity and increasing serum MKP-1 levels in women, and decreasing T levels in perimenopausal women. PMID: 26176177
  26. MKP-1 can attenuate tamoxifen-induced cell death through inhibiting the JNK signal pathway. PMID: 26329166
  27. Progesterone acts via GR to drive MKP-1 expression, which in turn inhibits IL-1beta-dependent c-Jun activation and COX-2 expression. PMID: 26280733
  28. Data show that sorafenib inhibited microRNA miR-101 expression and enhanced dual specificity phosphatase 1 (DUSP1) expression and lowered transforming growth factor beta I (TGF-beta) release in M2 macrophage, slowing macrophage-driven hepatocarcinoma. PMID: 26158762
  29. DUSP1 overexpression and inhibition of MAPKs prevented IL1B-induced expression of ZFP36, this was associated with increased TNF mRNA expression at 6 h, an effect that was predominantly due to elevated transcription. PMID: 26546680
  30. MKP-1 is a MAPK deactivator; thus, by controlling p38 MAPK phosphorylation status in a temporally distinct manner, MKP-1 ensures that TTP is expressed and made functional at precisely the correct time to repress cytokine expression. PMID: 25724669
  31. Wild-type but not mutant p53 transcriptionally upregulated DUSP1 via its DNA-binding domain. DUSP1 and p53 might collaborate to suppress tumors in hepatocarcinogenesis via a positive regulatory loop. PMID: 25617504
  32. Data (including data from studies in knockout mice) suggest AMPK activation (AMPKa1 or AMPKa2) suppresses inflammation in vascular smooth muscle cells by down-regulation of STAT1 signaling and up-regulation of MKP-1 (dual specificity phosphatase 1). PMID: 25858560
  33. DUSP1 is involved in the antiviral host defense mechanism against a HCV infection PMID: 25798824
  34. The proliferation of human cervical cancer cells was also inhibited by DUSP1 PMID: 25872469
  35. MKP1 recruits to the chromatin in response to DNA damage and correlates with the decrease of H3S10P, whereas MKP1 is released from chromatin during recovery phase of DNA damage response. PMID: 26111828
  36. Decreased DUSP1 might be a mechanism responsible for gefitinib resistance. PMID: 25593344
  37. Knockdown of MKP-1 abolished the miR-210-inhibition prevention of cell proliferation under hypoxia. PMID: 25044272
  38. PDE4 inhibitors augment antiinflammatory effects of beta2-agonists via increased MKP-1 expression in airway smooth muscle cells. PMID: 25296132
  39. Deregulation of EGR-1 and/or DUSP-1 in nasal epithelium could be responsible for the prolonged activated transcriptional state observed in vivo in allergic disease. PMID: 25638726
  40. DUSP1 upregulation is strongly linked to adiposity and that physical exercise modulates its expression. PMID: 25370852
  41. Data suggest that MKP-2 rather than MKP-1 is tamoxifen-regulated and that the elevated expression of MKP-2 in MCF7-TAMR cells potentially functions to restore tamoxifen sensitivity. PMID: 24658355
  42. Together, our results clearly establish the role of Dusp1 as a tumor suppressor gene that regulates cancer-associated inflammation. PMID: 25312268
  43. Combined inhibition of MKP1 and HER2 enhanced cell killing in breast cancer. Together, our findings identify a new mechanism of resistance in breast tumors and reveal MKP1 as a novel therapeutic target for radiosensitization. PMID: 25377473
  44. MKP-1 and MKP-2 stability is regulated by ERK-mediated phosphorylation through a degradation pathway independent of polyubiquitination PMID: 25204653
  45. DUSP1 promotes apoptosis in prostate cancer cells through the inhibition of p38 MAPK. PMID: 24080497
  46. DUSP1 expression is increased in obstructive sleep apnea and intermittent hypoxia PMID: 24267255
  47. Keratoconic corneas are distinguished with high expression of TGF-beta1 and DUSP1. PMID: 24339725
  48. Activating MAPK phosphatase-1 (MKP-1). PMID: 24086554
  49. DUSP1 plays a transient, often partial, role in the dexamethasone-dependent repression of certain inflammatory genes. PMID: 24692548
  50. MKP-1 promoted angiogenic and arteriogenic neovascular growth, potentially through dephosphorylation of histone H3 serine 10 on coding-region DNA to control transcription of angiogenic genes, such as fractalkine. PMID: 24578378

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Subcellular Location
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Tissue Specificity
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Database Links

HGNC: 3064

OMIM: 600714

KEGG: hsa:1843

STRING: 9606.ENSP00000239223

UniGene: Hs.171695

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