Recombinant Human E3 ubiquitin-protein ligase TRIM21(TRIM21)

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Code CSB-YP024457HU
Size US$1298
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Description

Recombinant Human E3 ubiquitin-protein ligase protein TRIM21 is a yeast-expressed full-length protein. It contains amino acid of 1-475 derived from human TRIM21 and has fused 6xHis-tags at the N-terminus. This TRIM21 protein is determined by SDS-PAGE, with purity greater than 90%. Recombinant protein has a calculated molecular weight of 56.2 kDa. The actual molecular mass increases slightly due to the post-modifications (PTMs). This product is available in stock, so you don't have to wait for the protein to be prepared when you buy it. Based on its major roles, this recombinant TRIM21 protein can be applied to the research area of epigenetics and nuclear signaling transduction.

E3 ubiquitin ligase TRIM21 is a predominant autoantigen in immune diseases and a regulator of innate immune signaling. In autoubiquitination, TRIM21 acts both as an E3 ligase and as a substrate. It also exerts antiviral functions. For instance, TRIM21 primarily facilitating the degradation of Hepatitis B virus (HBV) DNA Pol mainly through the K48-linked ubiquitin proteasome pathway via its RING domain, thus blocking HBV DNA replication. This may suggest a potential strategy for the treatment of HBV, infection of which will dvelope epatic fibrosis, cirrhosis even hepatocellular carcinoma.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names TRIM21
Uniprot No. P19474
Research Area Epigenetics and Nuclear Signaling
Alternative Names 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; Autoantigen Ro/SSA, 52-KD; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RNF81; Ro 52; Ro(SS-A); Ro52; RO52_HUMAN; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1; Sjogren syndrome type A antigen; SS-A; SSA; SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro); TRIM21; Tripartite motif protein TRIM21; Tripartite motif-containing 21; Tripartite motif-containing protein 21
Species Homo sapiens (Human)
Source Yeast
Expression Region 1-475aa
Target Protein Sequence MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 56.2kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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 Q&A
Q:

I am interested in the product CSB-YP024457HU. Would you mind answering the following questions?
1.Is this protein functionally active?
2.How is it made? from which system?
3.Is it properly folded?
4.Does it bind to Fc?

A:
Thanks for your inquiry. CSB-YP024457HU Recombinant Human E3 ubiquitin-protein ligase TRIM21(TRIM21)
1. We haven't tested the activity yet so can't 100% guarantee that this protein is active. The protein we provide is full length of mature protein, purified under mild conditions, which should have activity in theory.
2. This protein is expressed by Yeast expression system which can be folded and secreted expressed effectively in theory, but we can't 100% guarantee that it can be folded correct.
3. Antigenic sequence is within 200-239aa. The protein we provide is full-length which can bind to Fc in theory. This YP protein has been delivered for many times and some customers should have done binding experiment, but we haven't received any feedback yet.
4. We still have 80ug of inventory for this protein. The delivery form is liquid. The storage buffer is 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerole.

Target Data

Function E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses
Gene References into Functions
  1. the TRIM21 knockdown increases SALL1 levels, indicating that TRIM21 degrades both SALL1 and SALL4. PMID: 29511085
  2. The expression of TRIM21 mRNA and protein was significantly higher in Systemic lupus erythematosus PBMCs as compared to healthy controls. There was a correlation between TRIM21 mRNA expression and Systemic lupus erythematosus activities. PMID: 29385873
  3. Low TRIM21 expression is associated with RNA virus infections. PMID: 29743353
  4. TRIM21 positively regulated osteosarcoma cell proliferation. Overexpression of TRIM21 enhanced osteosarcoma cell tolerance toward various stresses. YWHAZ protein was identified as a novel interacting partner of TRIM21 and its expression levels were negatively regulated by TRIM21. PMID: 29673441
  5. expression increased in lesional psoriatic skin PMID: 27943421
  6. when misfolded tau assemblies enter the cell, they can be detected and neutralized via a danger response mediated by tau-associated antibodies and the cytosolic Fc receptor tripartite motif protein 21 (TRIM21) PMID: 28049840
  7. Taken together, the current study provides evidence of new function of Ro60/SSA in the development of cancer. It facilitates pancreatic cancer proliferation, migration and invasion. Therefore, it may represent a novel molecular target for the management of pancreatic cancer. PMID: 29274781
  8. We suggest that TRIM21 may be one of the factors associated with the "switching on" the proinflammatory programme in CD16(+) monocytes or monocyte-derived macrophages. PMID: 27773663
  9. Authors demonstrate that TRIM21 expression predicts survival in pancreatic cancer patients. This work highlights a novel mechanism of Par-4 regulation, and identifies a novel prognostic marker and potential therapeutic target for pancreatic cancer. PMID: 27830973
  10. TRIM21 role in TRAIL-induced apoptosis PMID: 27219672
  11. Our data suggest that patients with IIM, mainly DM, are characterized by a deficient expression of Ro52/TRIM21 in different PBMC subsets (CD4(+) lymphocytes and monocytes), along with lower K48-mediated ubiquitination, which is associated with a proinflammatory cytokine response. PMID: 27936488
  12. Significant relationships were found between clinical and laboratory manifestations of autoimmune and rheumatic diseases with different patterns of antibodies to anti-Ro52, anti-Ro60 and anti-La . PMID: 26725021
  13. downregulation of miR-1207-5p and miR-4695-3p expression may lead to increased TRIM21 levels in the minor salivary glands, which contributes to the development of Sjogren's syndrome PMID: 26888739
  14. The interaction of TRIM21 and LFG was analyzed by co-immunoprecipitation. To examine changes in regulatory processes, western blot analyses, real-time PCR, activity of apoptotic process and flow cytometric analyses were carried out. PMID: 26398169
  15. TRIM21 plays an essential role in p62-regulated redox homeostasis and may be a viable target for treating pathological conditions resulting from oxidative damage. PMID: 26942676
  16. TRIM21-induced exposure of the viral genome promotes sensing of DNA and RNA viruses by cGAS and RIG-I PMID: 26506431
  17. we prove that TRIM21 is a potential tumor suppressor in hepatocellular carcinoma and its low expression indicates poor prognosis PMID: 26055142
  18. Trim21 regulates Nmi-IFI35 complex-mediated inhibition of innate antiviral response PMID: 26342464
  19. TRIM20 and TRIM21 mediate precision autophagy, controlling the hub signaling machineries and key factors, inflammasome and type I interferon, directing cardinal innate immunity response systems in humans. PMID: 26347139
  20. This study elucidates a complex mechanism of step-wise ubiquitination and deubiquitination activities that allows contemporaneous innate immune signaling and neutralization by TRIM21. PMID: 26150489
  21. Anti-Ro52/TRIM21 antibodies are independently associated with the presence of interstitial lung disease and poor survival in systemic sclerosis. PMID: 26315678
  22. Endoplasmic reticulum stress causes autophagy and apoptosis leading to cellular redistribution of the SS-A and SS-B autoantigens in salivary gland epithelial cells of Sjogren's syndrome patients. PMID: 25845745
  23. These data extend the protective role of TRIM21 from viruses to bacteria and thereby strengthening the general role of antibody-dependent intracellular neutralisation in cellular immunity. PMID: 24920099
  24. Upregulation of SSA1 is associated with alcoholic and nonalcoholic steatohepatitis. PMID: 25526666
  25. TRIpartite motif 21 (TRIM21) differentially regulates the stability of interferon regulatory factor 5 (IRF5) isoforms. PMID: 25084355
  26. Report association of Anti-Ro/SSA-p200 antibodies with congenital heart block. PMID: 25327946
  27. Although protein expression levels were not affected significantly, the late up-regulation of Ro52/TRIM21 mRNA was accompanied by protein redistribution PMID: 25098814
  28. the up-regulation of Ro52 in ductal epithelium might be a triggering factor for disease progression in Sjogren's syndrome. PMID: 24673429
  29. Cytoplasmic sequestration of GMPS requires ubiquitylation by TRIM21. PMID: 24462112
  30. epitope peptide of the TRIM21 (TRIM: tripartite motif) autoantigen that is recognized by a polyclonal antibody was determined as assembling an "L-E-Q-L" motif on an alpha-helix PMID: 24094071
  31. Regulation of TRIM-21 expression occurs through an ERalpha-dependent mechanism, a pathway that was observed to be overactive in SLE patients. PMID: 24449583
  32. Autoantibodies to the RING domain of Ro52 significantly correlate with disease activity in systemic lupus erythematosus. PMID: 23554036
  33. Our data demonstrate that multiple IRFs tightly regulate expression of Trim21 in immune cells. PMID: 23975864
  34. This retrospective study supports the routine distinction of anti-SSA/Ro60 and anti-Ro52/TRIM21 due to their different clinical associations. PMID: 23039326
  35. Anti-TRIM21 antibodies were the second most common autoantibodies in this systemic sclerosis cohort. PMID: 22394602
  36. Data suggest that the identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. PMID: 23357050
  37. Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. PMID: 23455675
  38. Anti-Ro52 antibodies were closely associated with the main clinical, histopathological and immunological features of primary Sjogren's syndrome. PMID: 22704838
  39. analysis of a novel role for tyrosine phosphorylation in regulating the interaction with IRF3 and the activity of TRIM21 downstream of TLR3 and TLR4 PMID: 22479513
  40. The direct interaction between TRIM21 and neutralizing antibody is essential, as single-point mutations within the TRIM21-binding site in the Fc region of a potently neutralizing antibody impair virus neutralization. PMID: 22647693
  41. data suggest that Ro52/SSA is involved in death receptor-mediated apoptosis by regulating c-FLIP(L) PMID: 22288650
  42. These findings shed light on a new physiological role for Ro52 that is important to intracellular immunity. PMID: 22178074
  43. anti-Ro52 autoantibodies binding the RING domain of Ro52 may be actively involved in the pathogenesis of rheumatic autoimmune disease by inhibiting Ro52-mediated ubiquitination. PMID: 21862588
  44. FADD and TRIM21 together negatively regulate the late IFN-alpha pathway in response to viral infection. PMID: 21183682
  45. 60 kD Ro and nRNP A frequently initiate human lupus autoimmunity PMID: 20224770
  46. Cells possess a cytosolic IgG receptor, tripartite motif-containing 21 (TRIM21), which binds to antibodies with a higher affinity than any other IgG receptor in the human body. PMID: 21045130
  47. Results suggest that Ro52-mediated ubiquitination promotes the degradation of IRF7 following TLR7 and TLR9 stimulation. PMID: 20668674
  48. Ro52-mediated monoubiquitination is involved in the subcellular translocation of active IKK beta to autophagosomes. PMID: 20627395
  49. Importantly, the Ro52 cytoplasmic bodies are highly motile and are located along the microtubule network. These results suggest that the Ro52 cytoplasmic bodies are unidentified structures that are transported along the microtubule network. PMID: 20013343
  50. Ro52 down-regulates Tax-induced NF-kappaB signalling by monoubiquitinating IKKbeta and by reducing the level of Tax PMID: 19675099

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Subcellular Location Cytoplasm, Cytoplasmic vesicle, autophagosome, Nucleus, Cytoplasm, P-body
Protein Families TRIM/RBCC family
Tissue Specificity Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.
Database Links

HGNC: 11312

OMIM: 109092

KEGG: hsa:6737

STRING: 9606.ENSP00000254436

UniGene: Hs.532357

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