Recombinant Human E3 ubiquitin-protein ligase TRIM21(TRIM21)

In Stock
Code CSB-YP024457HU
Product Type Recombinant Protein
Size US$2010
Uniprot No. P19474
Lead Time 3-7 business days
Relevance E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Storage Buffer Tris-based buffer,50% glycerol
Alias 52 kDa Ro protein52 kDa ribonucleoprotein autoantigen Ro/SS-ARING finger protein 81Ro(SS-A)Sjoegren syndrome type A antigen ;SS-ATripartite motif-containing protein 21
Species Homo sapiens (Human)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY
Research Area Epigenetics and Nuclear Signaling
Source Yeast
Gene Names TRIM21
Protein Names Recommended name: E3 ubiquitin-protein ligase TRIM21 EC= 6.3.2.- Alternative name(s): 52 kDa Ro protein 52 kDa ribonucleoprotein autoantigen Ro/SS-A RING finger protein 81 Ro(SS-A) Sjoegren syndrome type A antigen Short
Expression Region 1-475aa
Tag Info N-terminal 6xHis-tagged
Mol. Weight 56.2kDa
Protein Description Full Length
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses
Subcellular Location Cytoplasm, Cytoplasmic vesicle, autophagosome, Nucleus, Cytoplasm, P-body
Protein Families TRIM/RBCC family
Tissue Specificity Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.
Database Links

HGNC: 11312

OMIM: 109092

KEGG: hsa:6737

STRING: 9606.ENSP00000254436

UniGene: Hs.532357

Q&A and Customer Reviews

Q&A
Q:

I am interested in the product CSB-YP024457HU. Would you mind answering the following questions?
1.Is this protein functionally active?
2.How is it made? from which system?
3.Is it properly folded?
4.Does it bind to Fc?

A:
Thanks for your inquiry. CSB-YP024457HU Recombinant Human E3 ubiquitin-protein ligase TRIM21(TRIM21)
1. We haven't tested the activity yet so can't 100% guarantee that this protein is active. The protein we provide is full length of mature protein, purified under mild conditions, which should have activity in theory.
2. This protein is expressed by Yeast expression system which can be folded and secreted expressed effectively in theory, but we can't 100% guarantee that it can be folded correct.
3. Antigenic sequence is within 200-239aa. The protein we provide is full-length which can bind to Fc in theory. This YP protein has been delivered for many times and some customers should have done binding experiment, but we haven't received any feedback yet.
4. We still have 80ug of inventory for this protein. The delivery form is liquid. The storage buffer is 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerole.
Customer Reviews

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