Recombinant Human Epididymal sperm-binding protein 1 (ELSPBP1)

Code CSB-YP856901HU
MSDS
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Source Yeast
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Code CSB-EP856901HU
MSDS
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Source E.coli
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Code CSB-EP856901HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856901HU
MSDS
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Source Baculovirus
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Code CSB-MP856901HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ELSPBP1
Uniprot No.
Alternative Names
E12; ELSPBP1; Epididymal secretory protein 12; Epididymal sperm-binding protein 1; ESPB1_HUMAN; hE12
Species
Homo sapiens (Human)
Expression Region
25-223
Target Protein Sequence
MHEECV FPFTYKGSVY FTCTHIHSLS PWCATRAVYN GQWKYCQSED YPRCIFPFIY RGKAYNSCIS QGSFLGSLWC SVTSVFDEKQ QWKFCETNEY GGNSLRKPCI FPSIYRNNVV SDCMEDESNK LWCPTTENMD KDGKWSFCAD TRISALVPGF PCHFPFNYKN KNYFNCTNEG SKENLVWCAT SYNYDQDHTW VYC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity.
Gene References into Functions
  1. HCV core protein represses E-cadherin expression by upregulating E12/E47 levels to induce EMT in HCV-associated HCC. PMID: 25817725
  2. These biochemical and functional data reveal RANBP16 and RANBP17 as novel regulators of E2A protein action, and demonstrate specific interaction of E12 with RANBP17. PMID: 20503194
  3. Sperm-binding fibronectin type II-module proteins are genetically linked and functionally related. PMID: 17307309
  4. characterize the assembly of a five-component complex containing TAL1, LMO2, Ldb1, E12, and DNA. The bHLH domains of TAL1 and E12 alone primarily formed helical homodimers, but together formed heterodimers, to which LMO2 bound with high affinity PMID: 17910069
Subcellular Location
Secreted.
Protein Families
Seminal plasma protein family
Tissue Specificity
Detected in cauda epididymidal fluid and on sperm membrane (at protein level).
Database Links

HGNC: 14417

OMIM: 607443

KEGG: hsa:64100

STRING: 9606.ENSP00000340660

UniGene: Hs.104894

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