Recombinant Human F-box/SPRY domain-containing protein 1 (FBXO45)

Code CSB-YP008510HU
MSDS
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Source Yeast
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Code CSB-EP008510HU
MSDS
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Source E.coli
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Code CSB-EP008510HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008510HU
MSDS
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Source Baculovirus
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Code CSB-MP008510HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FBXO45
Uniprot No.
Alternative Names
F box protein 45; F-box only protein 45; F-box/SPRY domain-containing protein 1; FBSP1_HUMAN; FBX45; fbxo45; hFbxo45
Species
Homo sapiens (Human)
Expression Region
2-286
Target Protein Sequence
AAPAPGAGA ASGGAGCSGG GAGAGAGSGS GAAGAGGRLP SRVLELVFSY LELSELRSCA LVCKHWYRCL HGDENSEVWR SLCARSLAEE ALRTDILCNL PSYKAKIRAF QHAFSTNDCS RNVYIKKNGF TLHRNPIAQS TDGARTKIGF SEGRHAWEVW WEGPLGTVAV IGIATKRAPM QCQGYVALLG SDDQSWGWNL VDNNLLHNGE VNGSFPQCNN APKYQIGERI RVILDMEDKT LAFERGYEFL GVAFRGLPKV CLYPAVSAVY GNTEVTLVYL GKPLDG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration. Plays a role in the regulation of neurotransmission at mature neurons. May control synaptic activity by controlling UNC13A via ubiquitin dependent pathway. Specifically recognizes TP73, promoting its ubiquitination and degradation.
Gene References into Functions
  1. Relatively low FBXO45 expression in gastric cancer tissues is associated with cancer progression and constitutes an independent prognostic factor PMID: 28011490
  2. Data show that epithelial-mesenchymal transition (EMT)-transcription factors can be dynamically degraded by an atypical ubiquitin E3 ligase complex Skp1-Pam-Fbxo45 (SPFFbxo45). PMID: 25460509
  3. Fbxo45 is the substrate-receptor subunit of a functional E3 ligase for Par-4 that has a critical role in cancer cell survival. PMID: 24992930
  4. Study found that the R108C mutation in FBXO45 is a rare variant with a modest effect on schizophrenia risk that may disrupt the structure and function of the FBXO45 protein PMID: 24878430
  5. FBXO45 gene consisting of 3 exons, and the vicinity of the transcriptional start site had several estrogen receptor binding consensus sequences. PMID: 16012741
  6. FBXO45 promotes the proteasome-dependent degradation of p73. PMID: 19581926

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Subcellular Location
Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, presynaptic cell membrane.
Protein Families
FBXO45/Fsn family
Database Links

HGNC: 29148

OMIM: 609112

KEGG: hsa:200933

STRING: 9606.ENSP00000310332

UniGene: Hs.169815

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