Recombinant Human Guanine nucleotide-binding protein-like 3(GNL3)

Code CSB-YP874819HU
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Source Yeast
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Code CSB-EP874819HU
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Source E.coli
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Code CSB-EP874819HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874819HU
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Source Baculovirus
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Code CSB-MP874819HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names GNL3
Uniprot No. Q9BVP2
Alternative Names C77032 ; E2 induced gene 3 protein; E2-induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; GNL3_HUMAN; Guanine nucleotide binding protein like 3 (nucleolar); Guanine nucleotide binding protein like 3; Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3 ; Nucleolar GTP-binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced
Species Homo sapiens (Human)
Expression Region 1-549
Target Protein Sequence MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKKR KLETNPDIKP SNVEPMEKEF GLCKTENKAK SGKQNSKKLY CQELKKVIEA SDVVLEVLDA RDPLGCRCPQ VEEAIVQSGQ KKLVLILNKS DLVPKENLES WLNYLKKELP TVVFRASTKP KDKGKITKRV KAKKNAAPFR SEVCFGKEGL WKLLGGFQET CSKAIRVGVI GFPNVGKSSI INSLKQEQMC NVGVSMGLTR SMQVVPLDKQ ITIIDSPSFI VSPLNSSSAL ALRSPASIEV VKPMEAASAI LSQADARQVV LKYTVPGYRN SLEFFTVLAQ RRGMHQKGGI PNVEGAAKLL WSEWTGASLA YYCHPPTSWT PPPYFNESIV VDMKSGFNLE ELEKNNAQSI RAIKGPHLAN SILFQSSGLT NGIIEEKDIH EELPKRKERK QEEREDDKDS DQETVDEEVD ENSSGMFAAE ETGEALSEET TAGEQSTRSF ILDKIIEEDD AYDFSTDYV
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.
Gene References into Functions
  1. GNL3 promotes the EMT in colon cancer by activating the Wnt/betacatenin signaling pathway. GNL3 is upregulated in colon cancer and plays an important role in tumor growth, invasion and metastasis. PMID: 28849076
  2. In addition, migration and invasion demonstrated significant inhibition in Nucleostemin-deficient cells. Furthermore, the knockdown of Nucleostemin dramatically suppressed xenograft progression in BALB/c nude mice. Our findings suggest that Nucleostemin is associated with malignancy in an ovarian cancer SKOV-3 cell line. PMID: 28412352
  3. Identified a positive correlation between up-regulation of nucleostemin and human glioma cell proliferation and that knocking-down nucleostemin alleviated glioma proliferation by reducing beta-Catenin transportation into the nucleus. PMID: 26607678
  4. Relative expression of GNL3 in synovial tissue and fluid samples was significantly higher in the osteoarthritic group as compared to the non-osteoarthritic group. PMID: 27066995
  5. The expression level of GNL3 was examined in 89 human gastric cancer samples using immunohistochemistry (IHC) staining. GNL3 in gastric cancer tissues was significantly upregulated compared with paracancerous tissues. PMID: 26722529
  6. Study demonstrated overexpression of NS in most hepatocellular carcinoma tissues compared with their matched surrounding tissues, and silencing NS promoted UV- and serum starvation-induced apoptosis of MHCC97H and Bel7402 cells. PMID: 26517370
  7. over-expression of GNL3 and SKA3 in the PC-3 human prostate cancer cell line decreased in vitro cell migration and invasion PMID: 26429724
  8. overexpression of NS suppressed ARF polyubiquitination by its E3 ligase Ubiquitin Ligase for ARF and elongated its half-life, whereas knockdown of NS led to the decrease of ARF levels PMID: 24769896
  9. Silence of nucleostemin inhibit cell proliferation through up-regulating p21 expression and nucleostemin may be involved in the pathogenesis of esophageal squamous cell carcinoma. PMID: 25699361
  10. It is concluded that nucleostemin expression could not be specific in a certain type of cells in colon cancer cell line HT29. PMID: 24762489
  11. Data shows that the NS protein expression status was positively correlated with both ER and HER2 status and was a powerful prognostic factor. PMID: 24650343
  12. Based on our results, NS overexpression seems to have a causative role in gastric tumorigenesis and/or progression, and it could be considered as a potential tumor marker for diagnosis, molecular classification PMID: 24763828
  13. Gene expression profiling analysis revealed that cell cycle regulators and nucleotide metabolism-related genes were highly enriched in a gene set associated with leukaemia-initiating capacity that we termed the 'leukaemia stem cell gene signature'. PMID: 24960197
  14. cis-acting regulatory polymorphisms acting on GNL3 and SPCS1 contribute to the OA association signal at chromosome 3p21, and these genes therefore merit further investigation. PMID: 24886551
  15. Nucleostemin can be used for tracking minimal residual disease and is a helpful guide for treatment. PMID: 24119536
  16. NS functions downstream of Myc as a rate-limiting regulator of cell proliferation and transformation. PMID: 22081066
  17. The nucleostemin(NS)could promote the recruitment of PML-IV to SUMOylated TRF1 in TA( ) and ALT cells. PMID: 22641345
  18. NS undergoes a ubiquitin- and MDM2-independent proteasomal degradation when intracellular GTP levels are markedly reduced PMID: 22318725
  19. Nucleostemin is expressed in Glioblastoma-cancer stem cells isolated from patient samples, and that its expression, conversely to what it has been described for ordinary stem cells, does not disappear when cells are differentiated. PMID: 22174890
  20. nucleostemin expression level correlated with TWIST expression level in esophageal cancer cell lines; identified that high nucleostemin proportion, TWIST intensity, and advanced pathological N stage were correlated with poor relapse-free survival PMID: 22050045
  21. NS inhibits TRF1 dimerization and shortens TRF1 association with the telomere. PMID: 22045740
  22. a complex composed of TERT, BRG1, and NS/GNL3L maintains the function of tumor initiating cells PMID: 21730156
  23. expression in ovarian tumors closely correlated with origination, progression, and grading PMID: 21892090
  24. NS is widely expressed in normal and neoplastic oral epithelial tissues, and is likely a marker of proliferation PMID: 21443540
  25. Apoptosis of HL-60 leukemia cells can be induced by silencing NS gene expression. PMID: 19379559
  26. Reactive oxygen species regulate nucleostemin oligomerization and protein degradation. PMID: 21242306
  27. Data show that knockdown of NS dramatically reduced the sphere-forming activity of MCF7 and MDA-MB-231 breast cancer cells. PMID: 21045149
  28. NS may promote the progression of prostate cancer by inhibiting the expression of p15, p16, and p18 in PC-3 cells. PMID: 20021940
  29. Nucleostemin is an important G1/S checkpoint regulator and it could regulate cell cycles via a p53-independent pathway in prostate cancer. PMID: 20664182
  30. Differential expression of NS, a stem cell marker, and its variants in different types of brain tumor are reported. PMID: 20572164
  31. The upregulation of nucleostemin ,mRNA frequently occurs in ESCC tissues and is associated with malignancy of human esophageal squamous tumors. nucleostemin is required for EGF and EGFR expression. PMID: 19823871
  32. Nucleostemin mRNA and protein are over-expressed in human esophageal squamous cell carcinoma. PMID: 18646696
  33. Data suggest that nucleostemin can help regulate the proliferation of both cancer cells and stem cells, and may play an important role in the growth regulation of gastric cancer, liver cancer and other cancers. PMID: 15112336
  34. nucleostemin has a role in proliferation and tumorigenesis of HeLa cells PMID: 15595646
  35. The nucleolar residence of nucleostemin involves a transient and a long-term binding by the basic and GTP-binding domains, and a dissociation mechanism mediated by the COOH-terminal region. PMID: 15657390
  36. analysis of nucleolar localization of stem cell protein nucleostemin PMID: 15857956
  37. Results implied that nucleostemin may play an important role in both tumorigenesis and transforming human embryonic bone marrow mesenchymal stem cells into F6 tumor cells. PMID: 16012751
  38. Transfected protein interacts with several proteins in COS cells. PMID: 16097049
  39. Using a multipotential adherent population of stem cells, nucleostemin was localized to the nucleoli and occurred in 43.3% of the cells. PMID: 16282439
  40. Expression widely occurs in normal and malignant renal tissues, and is likely a proliferation marker rather than a unique regulator of cell proliferation and survival in stem and cancer cells. PMID: 16670719
  41. In cultured cells, nucleostemin is expressed in a distinct population of epidermal cells from hair follicle kept in the presence of a feeder layer, intimating an association of nucleostemin expression with this type of epithelio-mesenchymal interaction PMID: 17000083
  42. Transgenic overexpression of nucleostemin increases the population doubling and decreases the senescence of mouse embryonic fibroblasts. PMID: 17000763
  43. A general view of the NS-target genes indicates the possible pathways in which NS plays its role in proliferation control in Hela cells PMID: 17310849
  44. These results demonstrate that, in the cells investigated, the level of NS is regulated by p14(ARF) and the control of the G1/S transition by NS operates in a p53-dependent manner. PMID: 17494866
  45. may be involved in carcinogenesis of the cervix and progression of cervical carcinoma PMID: 18419830
  46. These results suggest that a p53-dependent cell cycle checkpoint monitors changes of cellular NS levels via the impediment of MDM2 function. PMID: 18426907
  47. Nucleophosmin is a binding partner of nucleostemin in human osteosarcoma cells PMID: 18448670
  48. Nucleostemin expression in cardiomyocytes is induced by fibroblast growth factor-2 and accumulates in response to Pim-1 kinase activity. PMID: 18519946
  49. nucleoplasmic relocation of nucleostemin during nucleolar disassembly safeguards the G2-M transit and survival of continuously dividing cells by MDM2 stabilization and p53 inhibition. PMID: 19033382
  50. a novel role of nucleostemin in ribosome biogenesis. PMID: 19106111

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Subcellular Location Nucleus. Nucleus, nucleolus.
Protein Families TRAFAC class YlqF/YawG GTPase family
Tissue Specificity Increased levels in lung tissue in cancer patients.
Database Links

HGNC: 29931

OMIM: 608011

KEGG: hsa:26354

STRING: 9606.ENSP00000395772

UniGene: Hs.313544

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