Recombinant Human Killer cell immunoglobulin-like receptor 2DL4(KIR2DL4),partial

Code CSB-YP857457HU
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Source Yeast
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Code CSB-EP857457HU
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Source E.coli
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Code CSB-EP857457HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857457HU
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Source Baculovirus
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Code CSB-MP857457HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names KIR2DL4
Uniprot No. Q99706
Alternative Names CD_antigen=CD158d; CD158 antigen like family member D; CD158 antigen-like family member D; CD158d; G9P; KI2L4_HUMAN; Killer cell immunoglobulin like receptor 2DL4; Killer cell immunoglobulin like receptor; two domains; long cytoplasmic tail; 4; Killer cell immunoglobulin-like receptor 2DL4; Killer cell inhibitory receptor 103AS; Killer Ig receptor; KIR 103AS; KIR-103AS; KIR103; KIR103AS; KIR2DL4; MHC class I NK cell receptor KIR103AS; Natural killer cell inhibitory receptor; NK cell receptor; NK cell receptor; natural killer cell inhibitory receptor; OTTHUMP00000068612; OTTHUMP00000068614; OTTHUMP00000068615
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Receptor for non-classical major histocompatibility class Ib HLA-G molecules. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M). In decidual NK cells, binds peptide-bound HLA-G-B2M complex and triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells. Upon interaction with peptide-bound HLA-G-B2M, initiates signaling from the endosomal compartment leading to downstream activation of PRKDC-XRCC5 and AKT1, and ultimately triggering NF-kappa-B-dependent proinflammatory response.
Gene References into Functions
  1. The allelic diversity of the KIR2DL4 receptor locus showed no significant association with leukemia among ethnic Hans from southern China. PMID: 29419875
  2. Data show that most of the Langerhans cell histiocytosis (LCH)cases were positive for KIR2DL4, suggesting that KIR2DL4 may serve as a marker for LCH diagnosis. PMID: 28445138
  3. Data show the alignment of different KIR2DL4 receptor alleles with ADOMA. PMID: 27491653
  4. Serum levels of soluble HLA-G were similar in CMV- and CMV+ subjects but levels in culture supernatants were significantly higher in cells from CMV+ than from CMV- subjects stimulated with CMV antigens. The HLA-G ligand KIR2DL4 was mainly expressed on NK cells and CD56+ T cells with no differences between CMV+ and CMV- subjects. PMID: 28817184
  5. a woman's heterozygosity in HLA-G and LILRB1 might be an advantage for a success of reproduction, but the partner's heterozygosity in 9A/10A KIR2DL4 alleles might not PMID: 26973020
  6. Decreased level of KIR2DL4 rather than its single nucleotide polymorphism is correlated with the susceptibility of pre-eclampsia PMID: 26823774
  7. the KIR2DL4 on human mast cells facilitates HLA-G-expressing cancer invasion and the subsequent metastasis. PMID: 25735953
  8. Among 438 Polish people, a weak difference was seen between males and females in 9769 C/A genotypes and alleles. Fertile women had a high frequency of homozygosity for the defective 9A variant, so KIR2DL4 is not absolutely necessary for reproduction. PMID: 25818657
  9. The 9620 9A/10A (rs11410751) polymorphism in exon 7 did not influence the risk of NSCLC. PMID: 25855135
  10. Results show that HLA-E and KIR2DL4 demonstrate evidence of positive selection between human, chimpanzee and gorilla suggesting a main role for immunological adaptations linked to embryo deep invasion of the maternal endometrium during pregnancy. PMID: 25451741
  11. The unique functional properties of KIR2DL4 may be mediated by self-association of the receptor. PMID: 25759384
  12. Genetic polymorphism of KIR2DL4, a putative NK cell receptor for HLA-G, does not influence susceptibility to asthma. PMID: 24033084
  13. Abs to KIR2DL4 and HLA-G did not block NK cell IFN-gamma production. PMID: 24337374
  14. KIR2DL4 was found to directly interact with HS/heparin, and the D0 domain of KIR2DL4 was essential for this interaction. PMID: 24127555
  15. Beta Microglobulin-free HLA-G activates NK cells via engagement of KIR2DL4 receptors. PMID: 23228393
  16. Sustained activation through CD158d induced morphological changes in NK cell shape and size, and survival in the absence of cell-cycle entry, all hallmarks of senescence. PMID: 23184984
  17. Significant differences in the frequency of KIR genes were found between the two populations despite being separated by only 300 km PMID: 22320834
  18. Differential transcription factor use by the KIR2DL4 promoter under constitutive and IL-2/15-treated conditions. PMID: 22467658
  19. KIR2DL4 gene may not be essential for fertility, as shown in a fertile Caucasian woman missing KIR2DL4 [case report] PMID: 21623736
  20. Decreased KIR2DL4 expression in placenta may participate in the pathogenesis of pre-eclampsia. PMID: 19134329
  21. The frequencies of alleles of killer cell immunoglobulin-like receptor genes, KIR3DL3 and KIR3DL2, and the carrier frequency of KIR2DL4 alleles have been determined from a population of African Americans by DNA sequencing of the coding regions. PMID: 21607693
  22. The expression of KIR2DL4 on NK cells in acute rejection group was statistically lower than that in stable kidney function group after renal transplantation. PMID: 21092455
  23. By promoting polyubiquitylation and degradation, E3 ubiquitin ligase Triad3A is identified as an interaction partner for the KIR2DL4 cytoplasmic domain. PMID: 21270397
  24. The sequential requirement for DNA-dependent protein kinase, Akt, and NF-kappaB in signaling by CD158d delineates a previously uncharacterized endosomal signaling pathway for a proinflammatory response in natural killer cells. PMID: 20179272
  25. KIR2DL4 itself is not associated with preeclampsia, but it may modulate the effect of HLA-G*0106 on risk for preeclampsia. PMID: 19700612
  26. The single tyrosine-based inhibitory motif (ITIM)in the cytoplasmic domain of KIR2DL4 efficiently inhibits natural cytotoxicity responses and recruits SHP-2 protein tyrosine phosphatase (but not SHP-1) to the 2DL4 cytoplasmic domain in NK cells. PMID: 11994457
  27. KIR2DL4 has the potential for both activating and inhibitory functions. PMID: 12055234
  28. NK clones express both 2DL4 alleles and either one or both alleles of the clonally restricted KIR 3DL1 and 3DL2 genes. PMID: 12538663
  29. All genotypes seen included genes for KIR2DL4. PMID: 12559621
  30. NK cell receptor, KIR2DL4:HLA-G interaction is not essential for human reproduction. PMID: 12616484
  31. The promoter for KIR2DL4 is expressed by all natural killer (NK) cell clones and drives reporter gene expression only in NK cells. PMID: 12794136
  32. isolated 3 distinct KIR2DL4 allele clones in each individual with multiple copies of KIR3DL/S1; assumption that unequal crossover event occurred between differing KIR haplotypes resulting in the duplication of the 2DL4, 3DS1/3DL1 genes PMID: 12826375
  33. KIR2DL4 transmembrane exon genotype influences both levels of membrane expression and activation of cytotoxic function. PMID: 12902476
  34. Appropriate engagement of KIR2DL4 can selectively stimulate NK cells to elicit a strong IFN-gamma response, which has the potential to contribute significant biological benefits, but only in individuals capable of expressing this receptor. PMID: 14500636
  35. Specific amplification of the D0 & D2 domains permit PCR discrimination of KIR2DL4 alleles & their frequencies in various cell lines and human populations. PMID: 14700593
  36. 4.5% of the individuals of a Caucasoid population bear a recombinant allele of KIR3DP1, officially designed KIR3DP1*004, that associates tightly with gene duplications of KIR3DP1, KIR2DL4 and KIR3DL1/KIR3DS1 PMID: 15580659
  37. report that ILT2 receptor, ILT3 receptor, ILT4 receptor, and KIR2DL4 receptor expression is up-regulated by HLA-G histocompatibility antigen in antigen-presenting cells, natural killer cells, and T cells PMID: 15670976
  38. evidence that FcepsilonRI-gamma (gamma) associates with 2DL4 to promote surface expression and provide signal transducing function. PMID: 15778339
  39. an inhibitory element was identified in the KIR2DL4 promoter and an activating element was found in the KIR3DL3 promoter; AML-2 acts as a repressor of expression of both KIR2DL4 and KIR3DL3 in mature NK cells PMID: 15778373
  40. KIR2DL4 alleles may explain the high frequency of this variation in the population. PMID: 15853895
  41. KIR2DL4 was constitutively internalized into Rab5-positive compartments in NK cells via dynamin-dependent process. Chemokine secretion induced by KIR2DL4 transfection into 293T cells occurred only with recombinant forms of KIR2DL4 trafficked to endosomes. PMID: 16366734
  42. New insights are provided into the mechanisms by which genetic polymorphism of the KIR2DL4 gene influences its expression. PMID: 17171757
  43. 8 known KIR2DL4 alleles were observed in individuals involved in bone marrow transplantation; 3 new alleles, KIR2DL4*00203, *00502, *0080104, differering from known alleles at the nucleotide but not the protein sequence level were also identified PMID: 17610421
  44. Three new alleles of KIR2DL4 have been identified, among those one allele showed alternatively spliced products. PMID: 18082267
  45. Signaling pathways are specifically activated by engagement of killer receptor KIR2DL4 transmembrane association with the FcepsilonRI-gamma (FCER1G) receptor in natural killer (NK) cells. PMID: 18292514
  46. Human leukocyte antigen-G, a ligand for the natural killer receptor KIR2DL4, is expressed by eutopic endometrium only in the menstrual phase. PMID: 18314122
  47. Increased competency of T cells to express KIR2DL4 with aging is conferred by a selective increase in H3-Lys 4 dimethylation and limited DNA demethylation PMID: 18586981
  48. Whereas 26% of the study population has a genotype corresponding to a lack of any functional membrane-bound form of the molecule, no association of the KIR2DL4 transmembrane alleles with susceptibility to MS was observed. PMID: 19304328
  49. Cross-linking with anti-LILRB1 on CD14(+) macrophages or anti-KIR2DL4 on CD56(+) NK cells resulted in up-regulation of a small subset of mRNAs including those for IL-6, IL-8, and TNFalpha PMID: 19304799
  50. Data showed that the CD70, perforin and KIR2DL4 promoters are demethylated in CD4(+)CD28(-) T cells, and that DNA methyltransferase 1 (Dnmt1) and Dnmt3a levels are decreased in this subset. PMID: 19394279

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Subcellular Location Cell membrane; Single-pass type I membrane protein. Early endosome membrane.
Protein Families Immunoglobulin superfamily
Tissue Specificity Expressed in decidual NK cells and innate lymphoid cell type I (ILC1). Expressed in a subset of peripheral NK cells.
Database Links

HGNC: 6332

OMIM: 604945

KEGG: hsa:3805

UniGene: Hs.661219

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