Recombinant Human LIM and calponin homology domains-containing protein 1 (LIMCH1), partial

Code CSB-YP891463HU
MSDS
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Source Yeast
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Code CSB-EP891463HU
MSDS
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Source E.coli
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Code CSB-EP891463HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891463HU
MSDS
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Source Baculovirus
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Code CSB-MP891463HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LIMCH1
Uniprot No.
Alternative Names
DKFZp434I0312; DKFZp686A01247; DKFZp686B2470; DKFZp686G18243; DKFZp686G2094; DKFZp781C1754; DKFZp781I1455; KIAA1102; LIM and calponin homology domains 1; LIM and calponin homology domains containing protein 1; LIM and calponin homology domains-containing protein 1; LIMC1_HUMAN; LIMCH 1; limch1; LIMCH1A; LMO7B; MGC72127; OTTHUMP00000218619
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.
Gene References into Functions
  1. Data suggest that LIMCH1 plays a positive role in regulation of NM-II activity through effects on MRLC during cell migration. PMID: 28228547
Subcellular Location
Cytoplasm, cytoskeleton, stress fiber.
Protein Families
LIMCH1 family
Database Links

HGNC: 29191

OMIM: 617750

KEGG: hsa:22998

STRING: 9606.ENSP00000316891

UniGene: Hs.335163

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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