Recombinant Human Laccase domain-containing protein 1 (LACC1)

Code CSB-YP814206HU
MSDS
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Source Yeast
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Code CSB-EP814206HU
MSDS
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Source E.coli
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Code CSB-EP814206HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP814206HU
MSDS
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Source Baculovirus
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Code CSB-MP814206HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LACC1
Uniprot No.
Alternative Names
C13orf31; Chromosome 13 open reading frame 31; CM031_HUMAN; Hypothetical protein LOC144811; UPF0124 protein C13orf31
Species
Homo sapiens (Human)
Expression Region
1-430
Target Protein Sequence
MAEAVLIDLF GLKLNSQKNC HQTLLKTLNA VQYHHAAKAK FLCIMCCSNI SYERDGEQDN CEIETSNGLS ALLEEFEIVS CPSMAATLYT IKQKIDEKNL SSIKVIVPRH RKTLMKAFID QLFTDVYNFE FEDLQVTFRG GLFKQSIEIN VITAQELRGI QNEIETFLRS LPALRGKLTI ITSSLIPDIF IHGFTTRTGG ISYIPTLSSF NLFSSSKRRD PKVVVQENLR RLANAAGFNV EKFYRIKTHH SNDIWIMGRK EPDSYDGITT NQRGVTIAAL GADCIPIVFA DPVKKACGVA HAGWKGTLLG VAMATVNAMI AEYGCSLEDI VVVLGPSVGP CCFTLPRESA EAFHNLHPAC VQLFDSPNPC IDIRKATRIL LEQGGILPQN IQDQNQDLNL CTSCHPDKFF SHVRDGLNFG TQIGFISIKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine, guanosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine, guanine and hypoxanthine. Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate. Also has adenosine deaminase activity. Acts as a regulator of innate immunity in macrophages by modulating the purine nucleotide metabolism, thereby regulating the metabolic function and bioenergetic state of macrophages. Enables a purine nucleotide cycle between adenosine and inosine monophosphate and adenylosuccinate that prevents cytoplasmic acidification and balances the cytoplasmic-mitochondrial redox interface. The purine nucleotide cycle consumes aspartate and releases fumarate in a manner involving fatty acid oxidation and ATP-citrate lyase activity. Participates in pattern recognition receptor (PRR)-induced cytokines in macrophages: associates with the NOD2-signaling complex and promotes optimal NOD2-induced signaling, cytokine secretion and bacterial clearance. Localizes to the endoplasmic reticulum upon PRR stimulation of macrophages and associates with endoplasmic reticulum-stress sensors, promoting the endoplasmic reticulum unfolded protein response (UPR). Does not show laccase activity.
Gene References into Functions
  1. mRNA expression levels of both HIF1A and LACC1 were upregulated in the skin lesions of individuals with leprosy. PMID: 29706348
  2. LACC1 is critical for amplifying PRR-induced outcomes, an effect that is attenuated by the LACC1 disease-risk variant. PMID: 28593945
  3. FAMIN is a peroxisome-associated protein with primary role(s) in macrophages and other immune cells, where its metabolic functions may be modulated by PPAR signaling events PMID: 27959965
  4. The results show that a frameshift mutation in LACC1 leads to a similar juvenile arthritis phenotype as previously described. This mutations strongly supports the significance of these gene variants in the development of juvenile arthritis PMID: 27881174
  5. LACC1 common SNPs represent genetic risk factors also for JIA and ulcerative colitis. PMID: 27098602
  6. There is an association of a mutation in LACC1 with a monogenic form of systemic juvenile idiopathic arthritis. PMID: 25220867

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Involvement in disease
Rheumatoid arthritis systemic juvenile (RASJ)
Subcellular Location
Cytoplasm. Nucleus. Endoplasmic reticulum. Peroxisome.
Protein Families
LACC1 family
Tissue Specificity
Ubiquitously expressed, with higher expression levels in immune-related tissues such as lymph nodes and spleen. Expressed in both intestinal and peripheral myeloid-derived cells.
Database Links

HGNC: 26789

OMIM: 604302

KEGG: hsa:144811

STRING: 9606.ENSP00000317619

UniGene: Hs.210586

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