Recombinant Human Mitochondrial ribonuclease P protein 3 (KIAA0391)

Code CSB-YP012187HU
MSDS
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Source Yeast
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Code CSB-EP012187HU
MSDS
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Source E.coli
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Code CSB-EP012187HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012187HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP012187HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIAA0391
Uniprot No.
Alternative Names
Kiaa0391; KIAA0391 gene; Mitochondrial ribonuclease P protein 3; Mitochondrial RNase P protein 3; Mitochondrial RNase P subunit 3; MRPP3; MRRP3_HUMAN; PRORP; Proteinaceous RNase P
Species
Homo sapiens (Human)
Expression Region
46-583
Target Protein Sequence
FSLKT MSPQNTKATN LIAKARYLRK DEGSNKQVYS VPHFFLAGAA KERSQMNSQT EDHALAPVRN TIQLPTQPLN SEEWDKLKED LKENTGKTSF ESWIISQMAG CHSSIDVAKS LLAWVAAKNN GIVSYDLLVK YLYLCVFHMQ TSEVIDVFEI MKARYKTLEP RGYSLLIRGL IHSDRWREAL LLLEDIKKVI TPSKKNYNDC IQGALLHQDV NTAWNLYQEL LGHDIVPMLE TLKAFFDFGK DIKDDNYSNK LLDILSYLRN NQLYPGESFA HSIKTWFESV PGKQWKGQFT TVRKSGQCSG CGKTIESIQL SPEEYECLKG KIMRDVIDGG DQYRKTTPQE LKRFENFIKS RPPFDVVIDG LNVAKMFPKV RESQLLLNVV SQLAKRNLRL LVLGRKHMLR RSSQWSRDEM EEVQKQASCF FADDISEDDP FLLYATLHSG NHCRFITRDL MRDHKACLPD AKTQRLFFKW QQGHQLAIVN RFPGSKLTFQ RILSYDTVVQ TTGDSWHIPY DEDLVERCSC EVPTKWLCLH QKT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic ribonuclease component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends. The presence of TRMT10C/MRPP1, HSD17B10/MRPP2 is required to catalyze tRNA molecules in their 5'-ends.
Gene References into Functions
  1. This study presents the crystal structure of human MRPP3, which features a remarkably distorted and hence non-productive active site that authors propose will switch to a fully productive state only upon association with MRPP1, MRPP2 and pre-tRNA substrate. PMID: 25953853
  2. using a genome-wide association study, found that posttranscriptional modification of functionally important sites in mitochondrial transfer RNAs (tRNAs) is under strong genetic control, largely driven by a missense mutation in MRPP3 PMID: 24763589
  3. Inhibition of mitochondrial RNase P by beta-amyloid is an unspecific effect and is not mediated by beta-amyloid interaction with SDR5C1. PMID: 23755257
  4. Haplotypes in KIAA0391 and PSMA6 genes is a genetic link for myocardial infarction and coronary artery disease. PMID: 19624571
Subcellular Location
Mitochondrion.
Protein Families
PPR family, P subfamily
Database Links

HGNC: 19958

OMIM: 609947

KEGG: hsa:9692

STRING: 9606.ENSP00000454657

UniGene: Hs.458487

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