Recombinant Human Pro-neuregulin-3, membrane-bound isoform(NRG3),partial

Code CSB-EP016079HU
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP016079HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NRG3.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP016079HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NRG3.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names NRG3
Uniprot No. P56975
Research Area Cancer
Alternative Names HRG3; Neuregulin 3; Neuregulin 3 like polypeptide; Neuregulin-3; Neuregulin3; NRG-3; Nrg3; NRG3_HUMAN; Pro neuregulin 3; pro NRG3; Pro-NRG3
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-360aa
Target Protein Sequence MSEGAAAASPPGAASAAAASAEEGTAAAAAAAAAGGGPDGGGEGAAEPPRELRCSDCIVWNRQQTWLCVVPLFIGFIGLGLSLMLLKWIVVGSVKEYVPTDLVDSKGMGQDPFFLSKPSSFPKAMETTTTTTSTTSPATPSAGGAASSRTPNRISTRLTTITRAPTRFPGHRVPIRASPRSTTARNTAAPATVPSTTAPFFSSSTLGSRPPVPGTPSTQAMPSWPTAAYATSSYLHDSTPSWTLSPFQDAASSSSSSSSSATTTTPETSTSPKFHTTTYSTERSEHFKPCRDKDLAYCLNDGECFVIETLTGSHKHCRCKEGYQGVRCDQFLPKTDSILSDPTDHLGIEFMESEEVYQRQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 53.8kDa
Protein Length Extracellular Domain
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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 Q&A
Q:

May I ask you for support in the following inquiry please?
It concerns the human recombinant Neuregulin proteins NRG2 and NRG3 (Cat# CSB-EP016079HU).
I would like to stimulate human cells in culture with these ligands, and check downstream signaling by immunoprecipitation and western blotting of target proteins. At the moment the source organism is probably irrelevant as long as the ligands are active when used for stimulation of cells in culture.
Have you done any such tests with these growth factors?

A:
Very nice to receive your inquiry. Code: CSB-EP016079HU
Name: Recombinant Human Pro-neuregulin-3, membrane-bound isoform(NRG3),partial
Expression Region: 1-360aa, extracellular domain
Tag Info: N-terminal 6xHis-SUMO-tag
Target Protein Sequence:

MSEGAAAASPPGAASAAAASAEEGTAAAAAAAAAGGGPDGGGEGAAEPPRELRCSDCIVWNRQQTWLCVVPLFIGFIGLGLSLMLLKWIVVGSVKEYVPTDLVDSKGMGQDPFFLSKPSSFPKAMETTTTTTSTTSPATPSAGGAASSRTPNRISTRLTTITRAPTRFPGHRVPIRASPRSTTARNTAAPATVPSTTAPFFSSSTLGSRPPVPGTPSTQAMPSWPTAAYATSSYLHDSTPSWTLSPFQDAASSSSSSSSSATTTTPETSTSPKFHTTTYSTERSEHFKPCRDKDLAYCLNDGECFVIETLTGSHKHCRCKEGYQGVRCDQFLPKTDSILSDPTDHLGIEFMESEEVYQRQ


We haven't tested the activity of this protein when it is used as ligand, and haven't found relevant literature reporting the activity of exogenous expression protein.
Here we provide the literature regarding this isolated protein's structure and function:
http://www.uniprot.org/uniprot/P56975/publication

Target Background

Function
(From Uniprot)
Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
Gene References into Functions
  1. No genetic correlation was found between Hirschsprung disease and the three SNPs at NRG3 (rs10748842, rs10883866 and rs6584400) PMID: 28256518
  2. Data show that Williams-Beuren syndrome transcription factor (WSTF) release was mediated by neuregulin-3 (NRG3) following KRASG12V expression in intestinal epithelial cells. PMID: 27449290
  3. These observations encourage investigation of the neurobiology of NRG3 isoforms and highlight inhibition of NRG3 signaling as a potential target for psychiatric treatment development. PMID: 27771971
  4. common variants of GABRG2, RELN and NRG3 and the GABRG2-RELN-PTCH1 interaction networks might confer altered susceptibility to Hirschsprung disease. PMID: 27889765
  5. these data suggest that Nrg3/ErbB4 signaling may be an important factor in nicotine dependence. PMID: 23999525
  6. This study demonstrated that genetic variants in the NRG3 gene play a role in alzheimer disease and revealed that SNPs in the NRG3 genes were more strongly associated with onset age of Alzherimer disease. PMID: 24061483
  7. An association between rs10748842 genotype in neuregulin 3 does not fit a simple inefficiency model of risk association in dorsolateral prefrontal cortex physiology. PMID: 24431462
  8. NRG3 may be a susceptibility gene for Hirschsprung's disease in a Chinese population. PMID: 23315268
  9. Neuregulin 3 rs6584400 genetic carriers are associated with psychotic symptoms and attention performance in schizophrenia. PMID: 22831755
  10. no association of SNPs or haplotypes with risk of schizophrenia in Han Chinese population PMID: 22981155
  11. Our results suggest a role for NRG3 in HSCR etiology and provide insights into the relative contribution of structural variants in both syndromic and non-syndromic HSCR. PMID: 22589734
  12. NRG3 polymorphisms and haplotypes were similar between schizophrenia patients and healthy controls of Korean ethnicity PMID: 21762460
  13. Data show that rs1649942, which is located in the intron region of NM_001165973.1. (NRG3 neuregulin 3), was associated with poorer overall survival in women with optimally debulked tumors. PMID: 21705454
  14. Results suggest that NRG3 may be modulating early attentional processes for perceptual sensitivity and vigilance, with opposite effects in affected individuals and healthy controls. PMID: 20548296
  15. Data show that NRG3 is a schizophrenia susceptibility gene, provide quantitative insight into NRG3 transcription traits in the brain, and reveal a probable mechanistic basis for disease association. PMID: 20713722
  16. The NRG3 protein is a new post-translationally regulated isoform of neuregulin 3 expressed in the developing human central nervous system with a role in oligodendrocyte survival. PMID: 16478787
  17. In transgenic female mice, Nrg3 has a role in the initiation of mammary placodes along the body axis; alterations in morphogenesis and differentiation of other epidermal appendages are also observed, including the hair follicles. PMID: 17880691
  18. NRG3 was expressed in 42% of breast cancers studied. It was preferentially expressed in breast tumors co-expressing EGFR/HER4. PMID: 17962208
  19. The result of tis study supports that the NRG3 gene is a susceptibility gene for schizophrenia. PMID: 18708184
  20. NRG3 is primarily expressed in the central nervous system and is one of three paralogs of NRG1, a gene strongly implicated in SZ. PMID: 19118813
  21. Observational study and genome-wide association study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 18846501

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Subcellular Location Pro-neuregulin-3, membrane-bound isoform: Cell membrane, Single-pass type I membrane protein, Note=Does not seem to be active, SUBCELLULAR LOCATION: Neuregulin-3: Secreted, SUBCELLULAR LOCATION: Isoform 3: Cell membrane, Single-pass type I membrane protein
Protein Families Neuregulin family
Tissue Specificity Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, col
Database Links

HGNC: 7999

OMIM: 605533

KEGG: hsa:10718

STRING: 9606.ENSP00000361214

UniGene: Hs.125119

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