Recombinant Human RNA binding protein fox-1 homolog 2(RBFOX2)

Code CSB-EP019439HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019439HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RBFOX2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019439HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RBFOX2.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names RBFOX2
Uniprot No. O43251
Research Area Transcription
Alternative Names RNBP2; Fox 1 homologue; Fox-1 homolog B; FOX2; FXH; Hexaribonucleotide-binding protein 2; HNRBP2; RBFOX2; Repressor of tamoxifen transcriptional activity; RFOX2_HUMAN; RNA binding motif protein 9; RNA binding protein 9; RNA binding protein fox-1 homolog 2; RNA-binding motif protein 9; RNA-binding protein 9; RTA
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-380aa
Target Protein Sequence MEKKKMVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLISLPLVPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAAAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 44.4kDa
Protein Length Full Length of Isoform 10
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Tris-based buffer,50% glycerol
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.
Gene References into Functions
  1. Rbfox2 nonsense mutation is associated with hypoplastic left heart syndrome. PMID: 27485310
  2. Data show that while RBFOX1 and RBFOX2 do not mediate neuron-specific processing of UBE3A-ATS, these proteins play important roles in developing neurons and are not completely functionally redundant. PMID: 27146458
  3. Results showed that the expression patterns of these genes were indicative of the onset of EMT in in-vitro models, but not in tissue samples. However, the ratio between ESRP1 or ESRP2 and RBFOX2 significantly decreased during EMT and positively correlated with the EMT-specific phenotype in cell models. Low ESRP1/RBFOX2 ratio value was associated with a higher risk of metastasis in early breast cancer patients. PMID: 27911856
  4. Rbfox2 modulates the functions of vascular CaV1.2 calcium channel by dynamically regulating the expressions of alternative exons 9* and 33, which in turn affects the vascular myogenic tone. PMID: 28993448
  5. RBFOX2 dysregulation by dominant-negative RBFOX2 is an early pathogenic event in diabetic hearts. PMID: 27239029
  6. RBFox2 interactis with chromatin in a nascent RNA-dependent manner. RBFox2 inactivation eradicates PRC2 targeting on the majority of bivalent gene promoters and leads to transcriptional de-repression. PMID: 27211866
  7. Some of the widespread cellular functions of Rbfox2 protein are attributable to regulation of miRNA biogenesis, and might include the mis-regulation of miR-20b and miR-107 in cancer and neurodegeneration. PMID: 27001519
  8. RBFOX proteins can facilitate the splicing of micro-exons. We also found that PTBP1 likely regulates the inclusion of micro-exons, possibly by repressing the inclusion of micro-exons that are enhanced by RBFOX proteins and other splicing factors.[RBFOX] PMID: 25524026
  9. CPSF2 and SYMPK, are RBFOX2 cofactors for both inclusion and exclusion of internal exons. PMID: 25921069
  10. RBFOX2 SNPs showed evidence for effects across multiple reading and language traits. PMID: 25065397
  11. Results show that the conserved Rbfox2 RNA binding protein regulates 30% of the splicing transitions observed during myogenesis and is required for the specific step of myoblast fusion. PMID: 25087874
  12. MBNL1 and RBFOX2 cooperate to establish a splicing programme involved in pluripotent stem cell differentiation. PMID: 24048253
  13. RBFOX2 polymorphism is associated with breast cancer. PMID: 23143756
  14. RBFOX2 drives mesenchymal tissue-specific splicing in both normal and cancer tissues. PMID: 23149937
  15. FOX-2 is involved in splicing of ataxin-2 transcripts and that this splicing event is altered by overexpression of ataxin-1 PMID: 22666429
  16. functional significance of EMT-associated alternative splicing depletion of RBFOX2 in mesenchymal cells PMID: 21876675
  17. This study characterizes the mechanism by which RBFOX2 regulates protein 4.1R exon 16 splicing through the downstream intronic element UGCAUG. PMID: 22083953
  18. the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism PMID: 21747913
  19. Fox-2 plays an integral role in the regulation of LH2 splicing and knockdown of Fox-2 may suggest a novel approach to strategies directed against scleroderma. PMID: 20131247
  20. Fox-1 and Fox-2 isoforms specifically activate splicing of neuronally regulated exons, which requires UGCAUG enhancer elements PMID: 16260614
  21. Fox-1 and Fox-2 splicing factors have roles in alternative splicing of protein 4.1R PMID: 16537540
  22. Fox-1/Fox-2 proteins block prespliceosome complex formation at two distinct steps through binding to two functionally important UGCAUG elements. PMID: 18573872
  23. These results establish hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance their ability to antagonize ASF/SF2. PMID: 18573884
  24. predict thousands of Fox-1/2 targets with conserved binding sites, at a false discovery rate of approximately 24%, including many validated experimentally, suggesting a surprisingly extensive splicing regulatory networks PMID: 18794351
  25. These findings suggest that FOX2 functions as a critical regulator of a splicing network, and that FOX2 is important for the survival of human embryonic stem cells. PMID: 19136955

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Subcellular Location Nucleus, Cytoplasm
Database Links

HGNC: 9906

OMIM: 612149

KEGG: hsa:23543

STRING: 9606.ENSP00000413035

UniGene: Hs.282998

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