RBFOX2 Antibody

Datasheet

Code	CSB-PA019439LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBFOX2 antibody at 6μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 41, 38, 40, 48, 44 kDa Observed band size: 42 kDa IHC image of CSB-PA019439LA01HU diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA019439LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA019439LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RBFOX2 Polyclonal antibody

Uniprot No.

O43251

Target Names

RBFOX2

Alternative Names

ibody; Fox 1 homologue antibody; Fox-1 homolog B antibody; FOX2 antibody; FXH antibody; Hexaribonucleotide-binding protein 2 antibody; HNRBP2 antibody; RBFOX2 antibody; Repressor of tamoxifen transcriptional activity antibody; RFOX2_HUMAN antibody; RNA binding motif protein 9 antibody; RNA binding protein 9 antibody; RNA binding protein fox-1 homolog 2 antibody; RNA-binding motif protein 9 antibody; RNA-binding protein 9 antibody; RTA antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human RNA binding protein fox-1 homolog 2 protein (8-110AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RBFOX2 Antibody (Product code: CSB-PA019439LA01HU) is Non-conjugated. For RBFOX2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA019439LB01HU	RBFOX2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019439LC01HU	RBFOX2 Antibody, FITC conjugated
Biotin	CSB-PA019439LD01HU	RBFOX2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.

Gene References into Functions

Rbfox2 nonsense mutation is associated with hypoplastic left heart syndrome. PMID: 27485310
Data show that while RBFOX1 and RBFOX2 do not mediate neuron-specific processing of UBE3A-ATS, these proteins play important roles in developing neurons and are not completely functionally redundant. PMID: 27146458
Results showed that the expression patterns of these genes were indicative of the onset of EMT in in-vitro models, but not in tissue samples. However, the ratio between ESRP1 or ESRP2 and RBFOX2 significantly decreased during EMT and positively correlated with the EMT-specific phenotype in cell models. Low ESRP1/RBFOX2 ratio value was associated with a higher risk of metastasis in early breast cancer patients. PMID: 27911856
Rbfox2 modulates the functions of vascular CaV1.2 calcium channel by dynamically regulating the expressions of alternative exons 9* and 33, which in turn affects the vascular myogenic tone. PMID: 28993448
RBFOX2 dysregulation by dominant-negative RBFOX2 is an early pathogenic event in diabetic hearts. PMID: 27239029
RBFox2 interactis with chromatin in a nascent RNA-dependent manner. RBFox2 inactivation eradicates PRC2 targeting on the majority of bivalent gene promoters and leads to transcriptional de-repression. PMID: 27211866
Some of the widespread cellular functions of Rbfox2 protein are attributable to regulation of miRNA biogenesis, and might include the mis-regulation of miR-20b and miR-107 in cancer and neurodegeneration. PMID: 27001519
RBFOX proteins can facilitate the splicing of micro-exons. We also found that PTBP1 likely regulates the inclusion of micro-exons, possibly by repressing the inclusion of micro-exons that are enhanced by RBFOX proteins and other splicing factors.[RBFOX] PMID: 25524026
CPSF2 and SYMPK, are RBFOX2 cofactors for both inclusion and exclusion of internal exons. PMID: 25921069
RBFOX2 SNPs showed evidence for effects across multiple reading and language traits. PMID: 25065397
Results show that the conserved Rbfox2 RNA binding protein regulates 30% of the splicing transitions observed during myogenesis and is required for the specific step of myoblast fusion. PMID: 25087874
MBNL1 and RBFOX2 cooperate to establish a splicing programme involved in pluripotent stem cell differentiation. PMID: 24048253
RBFOX2 polymorphism is associated with breast cancer. PMID: 23143756
RBFOX2 drives mesenchymal tissue-specific splicing in both normal and cancer tissues. PMID: 23149937
FOX-2 is involved in splicing of ataxin-2 transcripts and that this splicing event is altered by overexpression of ataxin-1 PMID: 22666429
functional significance of EMT-associated alternative splicing depletion of RBFOX2 in mesenchymal cells PMID: 21876675
This study characterizes the mechanism by which RBFOX2 regulates protein 4.1R exon 16 splicing through the downstream intronic element UGCAUG. PMID: 22083953
the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism PMID: 21747913
Fox-2 plays an integral role in the regulation of LH2 splicing and knockdown of Fox-2 may suggest a novel approach to strategies directed against scleroderma. PMID: 20131247
Fox-1 and Fox-2 isoforms specifically activate splicing of neuronally regulated exons, which requires UGCAUG enhancer elements PMID: 16260614
Fox-1 and Fox-2 splicing factors have roles in alternative splicing of protein 4.1R PMID: 16537540
Fox-1/Fox-2 proteins block prespliceosome complex formation at two distinct steps through binding to two functionally important UGCAUG elements. PMID: 18573872
These results establish hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance their ability to antagonize ASF/SF2. PMID: 18573884
predict thousands of Fox-1/2 targets with conserved binding sites, at a false discovery rate of approximately 24%, including many validated experimentally, suggesting a surprisingly extensive splicing regulatory networks PMID: 18794351
These findings suggest that FOX2 functions as a critical regulator of a splicing network, and that FOX2 is important for the survival of human embryonic stem cells. PMID: 19136955

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Subcellular Location

Nucleus. Cytoplasm.

Database Links

HGNC: 9906

OMIM: 612149

KEGG: hsa:23543

STRING: 9606.ENSP00000413035

UniGene: Hs.282998

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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