Recombinant Human Serine/threonine-protein kinase DCLK1(DCLK1)

Code CSB-YP006548HU
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Source Yeast
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Code CSB-EP006548HU
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Source E.coli
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Code CSB-EP006548HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006548HU
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Source Baculovirus
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Code CSB-MP006548HU
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Calcium/calmodulin-dependent protein kinase type I-like CPG16; CL1; CLICK1; Cpg16; DCDC3A; Dcl; Dclk; Dclk1; DCLK1_HUMAN; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1; KIAA0369; Serine/threonine-protein kinase DCAMKL1; Serine/threonine-protein kinase DCLK1
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Protein Length
Full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Gene References into Functions
  1. DCLK1 plays an important role in cancer progression and is involved in the regulation of miR-200c expression. PMID: 29656186
  2. The expression of DCLK1 was found in low-grade adenomas and increased with worsening severity of dysplasia. DCLK1 expression was highly observed in advanced adenomas, which had a clinically higher malignant potential. PMID: 29475922
  3. High DCLK1 expression is associated with malignant pleural mesothelioma. PMID: 28560410
  4. On the basis of these data, FOXD3 is a potent repressor of DCLK1-S expression in normal cells; loss of FOXD3 in hCCCs/hCRCs allows upregulation of DCLK1-S, imparting a potent invasive potential to the cells PMID: 28851816
  5. Expression levels of phosphorylated cdc25A (p-cdc25A) and phosphorylated Chk1 (p-Chk1), belonging to the ATR pathway, were decreased by treatment with Dclk1 inhibitor LRRK2-IN-1 (LRRK), indicating Dclk1 involvement in the ATR pathway PMID: 29048622
  6. In rectal cancer patients treated with preoperative chemoradiotherapy (CRT), DCLK1 expression was significantly correlated with lymph node metastasis and poor cancer-specific survival. However, in patients treated without preoperative therapy, no such correlation was found. DCLK1 expression can be associated with lymph node metastasis and poor cancer-specific survival in rectal cancer patients who receive CRT. PMID: 29924454
  7. Pancreatic ductal adenocarcinoma patients with DCLK1-positive tumors had significantly shorter survival times than those with DCLK1-negative tumors. PMID: 28883702
  8. DCLK1 plays crucial role in the regulation of epithelial-mesenchymal transition.DCLK1 is highly expressed in pancreatic neuroendocrine tumor. PMID: 28179411
  9. DCLK1 is a prognostic biomarker for shortened survival. Secondly, through inhibition of DCLK1, it may serve as a therapeutic target as well PMID: 28351564
  10. DCLK1 has been identified as a potential target for miR-448 to regulate lung squamous cell carcinoma cells growth. PMID: 28320089
  11. Generated a (doublecortin like kinase 1) DCLK1-S-specific polyclonal antibody (S-isoform specific), and studied its use in screening of colon cancer after colonoscopy. PMID: 28414327
  12. DCLK1 expression was observed in tumor cells in patients with pathological stage I non-small cell lung cancer (NSCLC)and was correlated with adverse prognosis, especially in patients with adenocarcinoma. DCLK1 may be a potential new therapeutic target PMID: 28382517
  13. Biochemical and structural characterization of DCLK1 allowed for the mapping of cancer-causing mutations within the kinase domain. PMID: 27545623
  14. Hence, we outline and discuss emerging evidence that strongly supports tuft cells as key regulatory cells in the complex network of the intestinal microenvironment. [review] PMID: 28684459
  15. Our results indicate that Dclk1 is essential in advancing intestinal tumorigenesis. Knocking down Dclk1 decreases tumor stemness and progression and is thus predicted to regulate pro-survival signaling and tumor cell pluripotency. PMID: 28148261
  16. The univariate and multivariate analyses suggested DCAMKL1 protein overexpression was an unfavorable prognostic factor in bladder cancer patients. In conclusion, DCAMKL1 is an independent poor prognostic factor for bladder cancer patients. PMID: 28621231
  17. DCLK1 induction and its overexpression following hepatic injury are likely to contribute to tumorigenesis including maintenance and dissemination of tumor cells in circulation. PMID: 27694285
  18. first evidence for the suppressive activity of miR-613 in hepatocellular carcinoma, which is causally linked to targeting of DCLK1 PMID: 27049311
  19. MSX1 and DCLK1 might be used in colorectal cancer detection or as target of cancer therapies. PMID: 27966796
  20. Increased expression of stromal DCLK1 was detected in endometriotic tissue compared to endometriosis patient endometrium. Stromal expression of DCLK1 was increased in endometrium of endometriosis patients compared to controls. PMID: 27881125
  21. DCLK1 up-regulation may play a contributory role in colorectal cancer metastasis and poor prognosis via activation of EMT. DCLK1 may serve as an independent predictor for CRC prognosis. PMID: 27520310
  22. Results showed the mechanism in which miR-137 regulates the expression of DCLK1, and demonstrated the opposite expression patterns of miR-137/DCLK1 in human non-small cell lung carcinoma and colon cancer stem cells. PMID: 26747706
  23. DCLK1 promoter methylationis associated with lung cancer. PMID: 26311076
  24. DCLK1 expression could aid in the prognostication and management of breast cancer with neuroendocrine differentiation. PMID: 26621833
  25. DCLK1 controls these complex cellular signaling pathways to regulate hepatocellular carcinoma growth, and may be used as a prognostic biomarker. PMID: 26468984
  26. results provide new insights into the molecular mechanism of the hepatitis B/C-virus induced liver inflammation and tumorigenesis via DCLK1-controlled networks PMID: 25948779
  27. Data suggest that doublecortin and CaM kinase-like 1 protein short-transcripts (DCLK1-S) may represent an important target for preventing/inhibiting colon-cancers, and for eliminating colon-cancer-stem-cells (CSCs). PMID: 26447334
  28. DCLK1 is essential for the invasive and metastatic properties of pancreatic cancer stem cells. PMID: 26764906
  29. Data indicate that doublecortin-like kinase 1 (DCLK1) is overexpressed and has a unique methylation signature in renal clear cell carcinoma (RCC). PMID: 25605241
  30. Doublecortin-like kinase 1 is elevated serologically in pancreatic ductal adenocarcinoma and widely expressed on circulating tumor cells. PMID: 25723399
  31. Increased expression of DCLK1 was observed in the epithelium, stroma and plasma of patients with Barrett's esophagus and esophageal adenocarcinoma. PMID: 25283374
  32. Potential exist for DCLK1 as a colorectal cancer biomarker for early detection, but may also have clinical implications regarding the previously proposed therapy toward DCLK1-positive cancer cells. PMID: 24384857
  33. XMD8-92 treatment results in inhibition of DCLK1 and downstream oncogenic pathways. PMID: 24880079
  34. RNA interference-mediated silencing of DCLK1 triggers apoptotic cell death of colon cancer cells in vitro and in vivo. PMID: 24626093
  35. DCLK1 mRNA levels were considerably reduced during FLV treatment. PMID: 24260365
  36. DCLK1 regulates pluripotency and angiogenic factors via microRNA-dependent mechanisms in pancreatic cancer. PMID: 24040120
  37. Human pancreatic ductal adenocarcinoma cells contain morphologically and functionally distinct subpopulations, expressing DCLK1, that have cancer stem cell-like properties. PMID: 24096005
  38. DCAMKL1 represses osteoblast activation by antagonizing Runx2, the master transcription factor in osteoblasts. PMID: 23918955
  39. Dclk1 distinguishes between tumor and normal stem cells in the intestine. PMID: 23202126
  40. DCAMKL-1 marks a subset of colorectal stem cells, as well as a subset of entero-endocrine cells. PMID: 22749579
  41. the progressive increase of DCAMKL-1 expression in Barrett's esophagus from dysplasia to esophageal adenocarcinoma PMID: 21916995
  42. Several DCLK1 variants were associated with disease phenotypes for rs7989807 in intron 3 (combination of SCZ, ADHD and BP), intron 19 (SCZ+BP) and the 3'UTR (SCZ+BP). PMID: 22539971
  43. High DCAMKL1 is associated with HCV-induced hepatocarcinogenesis. PMID: 21937640
  44. Eight suggestive significant loci were detected with a series of genes expressed within the inner ear that underlie the auditory function, such as: DCLK1, PTPRD, GRM8, CMIP. PMID: 21493956
  45. Data show that siRNA-mediated knockdown of DCAMKL-1 in pancreatic cancer cells induced microRNA miR-200a, an EMT inhibitor, along with downregulation of EMT-associated transcription factors. PMID: 21285251
  46. Together, our results suggest that silencing the DCLK splice variants DCL and DCLK-long induces apoptosis in NB cells. PMID: 20228126
  47. genomic organization underlying the splice variants of DCLK and the expression profile of two splice variants affecting the kinase domain of DCLK and CPG16 (candidate plasticity gene 16). PMID: 11884394
  48. high-quality wild-type crystals of doublecortin-like kinase were obtained and a complete native data set was collected to 1.5 A resolution PMID: 12595708
  49. an examination of the binding sites on this protein PMID: 12692530

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Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
Tissue Specificity
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The ty
Database Links

HGNC: 2700

OMIM: 604742

KEGG: hsa:9201

STRING: 9606.ENSP00000255448

UniGene: Hs.507755

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