Recombinant Human Sialidase-3(NEU3),partial

Code CSB-EP891986HU
Size $419
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names NEU3
Uniprot No. Q9UQ49
Research Area Metabolism
Alternative Names NEU3Sialidase-3; EC 3.2.1.18; Ganglioside sialidase; Membrane sialidase; N-acetyl-alpha-neuraminidase 3
Species Homo sapiens (Human)
Source E.coli
Expression Region 2-428aa
Target Protein Sequence EEVTTCSFNSPLFRQEDDRGITYRIPALLYIPPTHTFLAFAEKRSTRRDEDALHLVLRRGLRIGQLVQWGPLKPLMEATLPGHRTMNPCPVWEQKSGCVFLFFICVRGHVTERQQIVSGRNAARLCFIYSQDAGCSWSEVRDLTEEVIGSELKHWATFAVGPGHGIQLQSGRLVIPAYTYYIPSWFFCFQLPCKTRPHSLMIYSDDLGVTWHHGRLIRPMVTVECEVAEVTGRAGHPVLYCSARTPNRCRAEALSTDHGEGFQRLALSRQLCEPPHGCQGSVVSFRPLEIPHRCQDSSSKDAPTIQQSSPGSSLRLEEEAGTPSESWLLYSHPTSRKQRVDLGIYLNQTPLEAACWSRPWILHCGPCGYSDLAALEEEGLFGCLFECGTKQECEQIAFRLFTHREILSHLQGDCTSPGRNPSQFKSN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 52.1kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.
Gene References into Functions
  1. This first demonstration of sialidase involvement in invasive potential of gliolastoma cells may point to NEU3 as an attractive treatment target of human gliomas PMID: 28760640
  2. effect of overexpression on EGFR downstream pathway activation and TKI targeted therapies sensitivity in non-small cell lung cancer cell lines PMID: 29088281
  3. The most potent compound tested targeted the human Neu4 isoenzyme, and was able to substantially reduce the rate of cell migration. We found that the lateral mobility of integrins was reduced by treatment of cells with Neu3, suggesting that Neu3 enzyme activity resulted in changes to integrin-co-receptor or integrin-cytoskeleton interactions PMID: 27344026
  4. NEU3 possesses the ability to interact with specific proteins, many of which are different in each cell compartment, supporting NEU3 involvement in the cell stress response, protein folding, and intracellular trafficking. PMID: 26987901
  5. findings identify NEU3 as a participant in head and neck squamous cell carcinoma progression PMID: 26470851
  6. Besides the already reported indirect EGFR activation through GM3, sialidase NEU3 could also play a role on EGFR activation through its desialylation. PMID: 25922362
  7. NEU3 promotes anchorage-independent growth and in vivo tumorigenicity. PMID: 25803810
  8. In the plasma membrane, NEU3 has a role in endocytosis and protein binding at the cell surface. PMID: 26251452
  9. Results indicate an essential contribution of NEU3 to tumorigenic potential through maintenance of stem-like characteristics of colon cancer cells by regulating Wnt signaling at the receptor level. PMID: 25810027
  10. The results provide strong evidence that the serum sialidase is, in fact, NEU3, and this subtype may, therefore, be a potential utility for novel diagnosis of human cancers PMID: 25652216
  11. Studies indicate that ganglioside-specific sialidase NEU3 cleaves sialic acids preferentially from gangliosides. PMID: 25408341
  12. Weak anchorage of NEU1 and NEU3 to the plasma membrane and their loss during erythrocyte life could be a tool to preserve the cellular sialic acid content to avoid early aging of erythrocyte and processes of cell aggregation in the capillaries. PMID: 22903576
  13. NEU3 is a key regulator of the beta1 integrin-recycling pathway and FAK/AKT signaling and demonstrate its crucial role in renal cell carcinomas malignancy. PMID: 23139422
  14. Data indicate that sialidases Neu1 and Neu3 are present on sperm, and their activity is required for capacitation and zona pellucida binding. PMID: 22989879
  15. Data show that NEU1 was immunolocalized to both the plasma membrane and the perinuclear region, and NEU3 was detected both in the cytosol and nucleus. PMID: 22403397
  16. These data suggest that NEU3 regulates tumor progression through AR signaling, and thus be a potential tool for diagnosis and therapy of androgen-independent prostate cancer. PMID: 21681193
  17. NEU3 is highly expressed to enhance malignant phenotypes including apoptosis inhibition in malignant melanomas. PMID: 21895867
  18. observed that trisaccharides with incorporated azide groups in the Neu5Ac residue at either C9 or the N5-Ac position were substrates, and in the case of the N5-azidoacetyl derivative, the activity was superior to that of GM3 PMID: 21675735
  19. Truncations at the N- or C-terminus of more than 10 residues abolished enzyme activity. PMID: 20511247
  20. NEU3 expression is diversely regulated by Sp1/Sp3 transcription factors binding to alternative promoters, which might account for multiple modulation of gene expression. PMID: 20518744
  21. Data show that Neu3 could represent a new potent biomarker in childhood ALL, to assess the efficacy of therapeutic protocols and to rapidly identify an eventual relapse. PMID: 19588508
  22. Neu3 functions as a caveolin-related signaling molecule within caveolin-rich microdomains PMID: 12011038
  23. up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression PMID: 12149448
  24. NEU3 participates in the control of insulin signaling via modulation of gangliosides and interaction with Grb2 PMID: 12730204
  25. Results show that the sialidase activity of human membrane type ganglioside sialidase (Neu3)is specific for gangliosides. PMID: 15179041
  26. Data show that the differentiation of monocytes into macrophages is associated with regulation of the expression of at least three distinct cellular sialidases, Neu1, 3, and 4, with specific up-regulation of the enzyme activity of only Neu1. PMID: 15885103
  27. NEU3 differentially regulates cell proliferation through integrin-mediated signalling depending on the extracellular matrix and, on laminins, NEU3 activated molecules often up-regulated in carcinogenesis. PMID: 16241905
  28. NEU3 activated by IL-6 exerts IL-6-mediated signaling, largely via the PI3K/Akt cascade, in a positive feedback manner and contributes to expression of a malignant phenotype in renal cell carcinoma PMID: 16428383
  29. NEU3 is able to mobilize to membrane ruffles in response to growth stimuli and activate the Rac-1 signaling by co-localization with Rac-1, leading to increased cell motility. PMID: 16765317
  30. The activity of Neu3 changed minimally in activated lymphocytes. PMID: 17028199
  31. NEU3 is an important regulator of insulin sensitivity and glucose tolerance. PMID: 17292733
  32. NEU3 may be an essential gene for cancer cell survival. PMID: 17334392
  33. Neu3 is up-regulated in Jurkat cells undergoing etoposide-induced apoptosis PMID: 17827720
  34. NEU3 is markedly up-regulated in many types of cancers including colon and renal carcinomas and suppresses apoptosis of cancer cells. PMID: 18023981
  35. Neu3 degrades membrane sialic acids resulting in downregulation of the PKC/ERKs/p38 MAPK signaling pathway, causing a decrease in CD41b surface antigen expression and inhibition of megakaryocytic differentiation of K562 cells. PMID: 18339327
  36. related to malignancy and may be potential targets for cancer diagnosis and therapy PMID: 18651674
  37. upon Neu3 silencing, K562 cells show a decrease in proliferation, propensity to undergo apoptosis, and megakaryocytic differentiation. PMID: 18820643
  38. increased CD15 expression is not due to de novo biosynthesis of CD15, but results predominantly from induction of alpha(2-3)-sialidase activity PMID: 18953356
  39. occurrence of Neu3 in the inner membrane and Neu1 in the outer membrane of the nuclear envelope PMID: 19686243

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Subcellular Location Cell membrane; Peripheral membrane protein. Membrane, caveola. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein.
Protein Families Glycosyl hydrolase 33 family
Tissue Specificity Highly expressed in skeletal muscle, testis, adrenal gland and thymus, followed by pancreas, liver, heart and thymus. Weakly expressed in kidney, placenta, brain and lung.
Database Links

HGNC: 7760

OMIM: 604617

KEGG: hsa:10825

STRING: 9606.ENSP00000294064

UniGene: Hs.191074

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