Recombinant Human Ubiquitin-conjugating enzyme E2 Q1 (UBE2Q1)

Code CSB-YP800227HU
MSDS
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Source Yeast
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Code CSB-EP800227HU
MSDS
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Source E.coli
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Code CSB-EP800227HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP800227HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP800227HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UBE2Q1
Uniprot No.
Alternative Names
Galactosyl transferase associated protein; GTAP; NICE 5; NICE5; OTTHUMP00000035378; PRO3094; Protein NICE 5; Protein NICE-5; UB2Q1_HUMAN; UBE2Q; Ube2q1; Ubiquitin carrier protein Q1; Ubiquitin conjugating enzyme E2 Q1; Ubiquitin conjugating enzyme E2Q (putative) 1; Ubiquitin conjugating enzyme E2Q 1; Ubiquitin conjugating enzyme E2Q family member 1; Ubiquitin protein ligase Q1; Ubiquitin-conjugating enzyme E2 Q1; Ubiquitin-protein ligase Q1
Species
Homo sapiens (Human)
Expression Region
1-422
Target Protein Sequence
MQQPQPQGQQ QPGPGQQLGG QGAAPGAGGG PGGGPGPGPC LRRELKLLES IFHRGHERFR IASACLDELS CEFLLAGAGG AGAGAAPGPH LPPRGSVPGD PVRIHCNITE SYPAVPPIWS VESDDPNLAA VLERLVDIKK GNTLLLQHLK RIISDLCKLY NLPQHPDVEM LDQPLPAEQC TQEDVSSEDE DEEMPEDTED LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD YLNGAVSGSV QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI CMELLTKQGW SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ SYKSLVQIHE KNGWYTPPKE DG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in hormonal homeostasis in females. Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation.
Gene References into Functions
  1. UBE2Q1 is a candidate oncogene involved in HCC development. PMID: 29027712
  2. hypomethylation of the UBE2Q1 gene promoter is a potential biomarker for detecting hepatitis B virus-associated hepatocellular carcinoma. PMID: 28592717
  3. UBE2Q1 hypermethylation is associated with Colorectal Cancer. PMID: 26745068
  4. overexpression of UBE2Q1 is associated with Breast Carcinoma. PMID: 25987028
  5. These data imply that UBE2Q1 is upregulated in liver cancer cell lines and tumorous samples and may play a role in the development of HCC. PMID: 25311764
  6. UBE2Q1 is differentially expressed in colorectal cell lines and shows overexpression in colorectal tumors. PMID: 24197692
  7. This result along with the real-time PCR data shows that the novel human gene, UBE2Q1, is expressed in human breast and may have implications for pathogenesis of breast cancer. PMID: 22167327
  8. we found somatic mutations of HERC2, HERC3, TRIP12, UBE2Q1 and UBE4B genes in gastric carcinoma and colorectal carcinomas with microsatellite instability PMID: 22124266

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Subcellular Location
Nucleus. Cell projection, filopodium. Cytoplasm, cytosol.
Protein Families
Ubiquitin-conjugating enzyme family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 15698

OMIM: 617429

KEGG: hsa:55585

STRING: 9606.ENSP00000292211

UniGene: Hs.607928

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