Product Details

Purity

>85% (SDS-PAGE)

Target Names

UL97

Uniprot No.

Q6SW46

Alternative Names

UL97Serine/threonine protein kinase UL97; EC 2.7.1.-

Species

Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine protein kinase that plays important roles in several processes including nuclear viral egress, viral replication or regulation of host cell cycle progression. Participates in the acquisition of tegument during virion morphogenesis in the nucleus. Redistributes the host nuclear lamina by phosphorylating cellular Lamins-A/C. Plays a role in viral DNA synthesis by phosphorylating the DNA polymerase processivity factor UL44. Stimulates host cell cycle to support viral DNA synthesis by phosphorylating host retinoblastoma/RB1 protein. Additional substrates have been identified including host EF1D or H2B. Phosphorylates also host SAMHD1 and thereby counteracts its antiviral effect by reducing its dNTP hydrolase activity.

Gene References into Functions

The pUL97 interaction domain of pp65 was defined (282-415). A putative cyclin bridge that enhances pUL97-pp65 interaction was identified. PMID: 29022869
Mutations detected in this study that led to amino acid substitutions in the IX region of the UL97 gene (A594V, E596K, and S604F) may affect the activity of the pUL97 enzyme, since this region corresponds to the binding site of the phosphotransferase to the viral DNA chain. PMID: 29768545
pUL97 shows differential affinities to human cyclins. PMID: 27548200
Rb family proteins are regulated by HCMV UL97 and IE1, which attests to the importance of modulating Rb family protein function in human cytomegalovirus-infected cells PMID: 28289097
The authors conclude that human cytomegalovirus can obtain deoxyribonucleotide triphosphates in the absence of Rb phosphorylation and that viral UL97 can contribute to the efficiency of DNA replication in an Rb phosphorylation-independent manner. PMID: 27440891
In vitro phosphorylation of pUL69 by CDK9 or pUL97 did not occur in a single site-specific manner, but at multiple sites. PMID: 26555090
Results show ganciclovir resistance for UL97-mutations I610T and E596Y while mutation E596D was drug-sensitive. PMID: 26209398
The authors postulate that the mechanism of cytomegalovirus pUL97-human cyclin B1 interaction is determined by an active pUL97 kinase domain. PMID: 26270673
An LXCXE motif in UL97 helps activate E2F-dependent promoters independently of its stimulatory effect on Rb phosphorylation. PMID: 26100623
The sequence domain of human cytomegalovirus pUL97 responsible for the interaction with cyclin T1 was between amino acids 231-280. PMID: 24351800
These results reveal differential properties of pUL97 isoforms that affect viral replication. PMID: 24522923
A new ganciclovir resistant UL97-mutation A613V was isolated and characterized. PMID: 24120366
Resistance of human cytomegalovirus to cyclopropavir maps to a base pair deletion in the open reading frame of UL97. PMID: 23817384
drug resistance, growth and autophosphorylation phenotypes of several unusual UL97 mutations in the kinase catalytic domain were characterized PMID: 23650173
Human cytomegalovirus pUL97 regulates the viral major immediate early promoter by phosphorylation-mediated disruption of histone deacetylase 1 binding. PMID: 23616659
A UL97-null mutant of strain TB40/E, which encodes a full-length ULb' region, exhibited replication defects. PMID: 23536674
pRb inactivation is insufficient to restore efficient viral nuclear egress of human cytomegalovirus in the absence of UL97. PMID: 23427156
A new mutation, C518Y, was identified in two kidney recipients, and this strain showed high-grade ganciclovir resistance by plaque reduction assay PMID: 23011309
A cysteine-to-glycine mutation at codon 592 (C592G) of UL97 confers ganciclovir resistance. PMID: 23090742
At the amino acid level, ten natural polymorphisms of UL97 were identified PMID: 21570426
UL97 can serve as a novel tool to probe the cellular mechanisms that contribute to the formation of aggregates in polyglutamine disorders. PMID: 20732421
UL97 D605E polymorphism has a high prevalence in immunocompetent Japanese infants and children. PMID: 21362026
These results define a dual role for UL97: a kinase-mediated function in the formation of the virus-induced assembly compartment and a nonkinase function enhancing the efficacy of viral tegumentation and release. PMID: 21248036
The extreme N terminus (large isoform) comprises a highly efficient, bipartite nuclear localization signal (amino acids 6-35). A second sequence conferring a less efficient mode of nuclear translocation was identified downstream of amino acid 74. PMID: 21084499
the impact of two different mutations found at the same UL97 codon on drug susceptibility and viral replicative capacity PMID: 20843736
Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1. PMID: 20686030
pUL97 stereoselectively phosphorylates cyclopropavir to its (+)-monophosphate. PMID: 20547817
Human cytomegalovius UL97 mutation occurrs in Chinese transplant recipients. This mutation may be regarded as a natural sequence variant. PMID: 17112867
To our knowledge for the first time, seven of 12 amino acid changes (F102L, D118V, M330T, T400A, R507P and C511R and I533V) in the UL97 gene of an isolate are herein reported. PMID: 19712701

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Subcellular Location

Virion.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, HCMV ganciclovir subfamily

Database Links

KEGG: vg:3077517

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Recombinant Human cytomegalovirus Serine/threonine protein kinase UL97 (UL97), partial

Product Details

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