Product Details

Purity

>85% (SDS-PAGE)

Target Names

NEC1

Uniprot No.

P10215

Alternative Names

NEC1; UL31; Nuclear egress protein 1

Species

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)

Expression Region

1-306

Target Protein Sequence

MYDTDPHRRG SRPGPYHGKE RRRSRSSAAG GTLGVVRRAS RKSLPPHARK QELCLHERQR YRGLFAALAQ TPSEEIAIVR SLSVPLVKTT PVSLPFCLDQ TVADNCLTLS GMGYYLGIGG CCPACNAGDG RFAATSREAL ILAFVQQINT IFEHRAFLAS LVVLADRHNA PLQDLLAGIL GQPELFFVHT ILRGGGACDP RLLFYPDPTY GGHMLYVIFP GTSAHLHYRL IDRMLTACPG YRFVAHVWQS TFVLVVRRNA EKPTDAEIPT VSAADIYCKM RDISFDGGLM LEYQRLYATF DEFPPP

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2. Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space. There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.

Gene References into Functions

UL31-mediated biological functions in HSV-1 infection cycle PMID: 26854290
The nuclear egress complex, made up of UL31 and UL34 functions as minimal virus-encoded membrane-budding machinery during nuclear egress and does not require additional cellular factors. PMID: 24916797
Binding of viral UL31 to host TMEM140 impedes formation of HSV-1 UL31-UL34 complex. PMID: 25036476
UL47 formed a complex(es) with HSV-1 proteins UL34, UL31, and/or Us3, which have all been reported to be critical for viral nuclear egress. PMID: 24522907
Authors hypothesize that the final 20 amino acids of pU(L)25 are required for pU(L)31 to associate with capsids. In the absence of pU(L)25 from the capsid, regions of capsid-associated pU(L)17 are bound by pU(L)31. PMID: 24453362
An interaction between a complex of pU(L)17/pU(L)25, termed the C capsid-specific complex (CCSC), and pU(L)31, a component of the nuclear egress complex (NEC), is reported. PMID: 21821792
These data suggest that pU(L)31 facilitates initiation of infection and/or accelerates the onset of viral gene expression in a manner that correlates with NF-kappaB activation and is independent of the transactivator ICP27. PMID: 21389131
Immunolocalization and coimmunoprecipitation experiments with truncated forms of pUL34 and pUL31 confirm that N-terminal sequences of pUL34 and a C-terminal domain of pUL31 mediate interaction but not nuclear membrane targeting. PMID: 20106917
amino acids 137 to 181 of the UL34 protein are (i) sufficient to mediate interactions with the UL31 protein in vitro and in vivo, (ii) necessary for the colocalization of UL31 and UL34 in infected cells, and (iii) essential for normal viral replication. PMID: 15731273
UL13 phosphorylates Us3 in infected cells and regulates UL34 and UL31 localization, either by phosphorylating Us3 or by a Us3-independent mechanism. PMID: 16415024
US3 kinase activity regulates HSV-1 capsid nuclear egress at least in part by phosphorylation of lamin A/C PMID: 17428859
Phosphorylation of UL31 is important during the primary envelopment of nucleocapsids. PMID: 19279109
Optimal recruitment of gD to both leaflets of the nuclear membrane and gM to the inner nuclear membrane requires UL34 and UL31. PMID: 19279119

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Subcellular Location

Host nucleus inner membrane.

Protein Families

Herpesviridae NEC1 protein family

Database Links

KEGG: vg:2703350

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Recombinant Human herpesvirus 1 Virion egress protein UL31 (UL31)

Product Details

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Target Background

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