Product Details

Purity

>85% (SDS-PAGE)

Uniprot No.

P03089

Alternative Names

; Major capsid protein VP1; Major structural protein VP1

Species

JC polyomavirus (JCPyV) (JCV)

Expression Region

1-354

Target Protein Sequence

MAPTKRKGER KDPVQVPKLL IRGGVEVLEV KTGVDSITEV ECFLTPEMGD PDEHLRGFSK SISISDTFES DSPNRDMLPC YSVARIPLPN LNEDLTCGNI LMWEAVTLKT EVIGVTSLMN VHSNGQATHD NGAGKPVQGT SFHFFSVGGE ALELQGVLFN YRTKYPDGTI FPKNATVQSQ VMNTEHKAYL DKNKAYPVEC WVPDPTRNEN TRYFGTLTGG ENVPPVLHIT NTATTVLLDE FGVGPLCKGD NLYLSAVDVC GMFTNRSGSQ QWRGLSRYFK VQLRKRRVKN PYPISFLLTD LINRRTPRVD GQPMYGMDAQ VEEVRVFEGT EELPGDPDMM RYVDKYGQLQ TKML

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.

Gene References into Functions

All viral populations were characterized by rearrangements within the noncoding regulatory region (NCCR) and 1 point mutation, S267L in the VP1 gene, suggestive of neurotropic strains. One patient with Progressive multifocal leukoencephalopathy (PML) had a single neurotropic strain with rearranged noncoding regulatory region (NCCR), and 1 patient had a single strain with small NCCR alterations. PMID: 28453853
found that deletion of nucleotides 376-396 results in decreased levels of viral DNA replication and a lack of VP1 expression. PMID: 25142442
Synthetic antibodies and peptides recognizing progressive multifocal leukoencephalopathy-specific point mutations in polyomavirus JC capsid viral protein 1. PMID: 25879139
These data show that capsid proteins of Mad-1 and WT3 JC Polyomavirus can both engage alpha2,6-linked sialic acid on the lactoseries tetrasaccharide c as well as multiple sialylated gangliosides. PMID: 25855729
This study demonstrated that the 5-fold VP1 pore is an important structural feature of JC polyomavirus and that minor modifications to this structure have significant impacts on infectious entry. PMID: 25609820
C80 of JCV Vp1 is required for Vp1 stability and pentamer formation, and C247 is involved in capsid assembly in the nucleus PMID: 24130786
These results demonstrate that viruses from progressive multifocal leukoencephalopathy patients with single point mutations in VP1 disrupt binding to sialic acid motifs and render these viruses noninfectious. PMID: 23760462
VP1 C terminus mutants were replication-competent and remains stable overtime in vitro. PMID: 21940415
mutations of JCV VP1 might favor progressive multifocal leukoencephalopathy onset; VP1 substitutions may favor JCV brain invasion by abrogation of sialic acid binding with peripheral cells while maintaining sialic acid-independent binding with brain cells PMID: 21628664
model of the interaction between VP1 and the sialic acid component of the human JCV receptor PMID: 15347668
frequent amino acid substitutions in the outer loops of VP1 of JCPyV associated with progressive multifocal leukoencephalopathy (PML) PMID: 15958683
A unique deletion was found in the C terminus may be instrumental in facilitating entry or replication of JCV into granule cell neurons. PMID: 16894191
A possible role for merkel cells Polyomavirus as an etiologic agent in the carcinogenesis of merkel cell carcinoma. PMID: 18593898
JCV VP1 loop mutations are associated with a favorable prognosis for PML PMID: 19043822
several amino acids on surface of VP1 display accelerated evolution in viral sequences isolated from progressive multifocal leukoencephalopathy patients but not healthy subjects; evidence that some mutations are involved in binding of sialic acid PMID: 19197354

Hide All

Subcellular Location

Virion. Host nucleus.

Protein Families

Polyomaviruses coat protein VP1 family

Database Links

KEGG: vg:1489518

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP355946JAK
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP355946JAK
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP355946JAK-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP355946JAK
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP355946JAK
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant JC polyomavirus Major capsid protein VP1

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage