Recombinant Lactococcus lactis subsp. lactis Probable N-acetylmuramidase (acmA)

Code CSB-YP880312LNG
MSDS
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Source Yeast
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Code CSB-EP880312LNG
MSDS
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Source E.coli
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Code CSB-EP880312LNG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880312LNG
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP880312LNG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
acmA
Uniprot No.
Alternative Names
acmA; LL0272; L68758Probable N-acetylmuramidase; EC 3.2.1.17; Autolysin; Lysozyme; Peptidoglycan hydrolase
Species
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Expression Region
58-439
Target Protein Sequence
ATN STEAFIESIA ASAKPVADSN GLYPSVMIAQ AILESNWGSS QLSRAPYYNL FGIQGTYQGK SVVFKTQEYL NGKWVTKDMP FRVYPSFNQS FQDNAYVLKT TNFGNGPYYA KAWRANAATY QAATAALTGK YATDPNYGAS LNRIISQYNL TRFDGASSAG TSNSGGSTAT NTNNNSNTSS TTYTVKSGDT LWGISQKYGI SVAQIQSANN LKSTVIYIGQ KLVLTTSSSS SNTNSSTSSG NSAGTTTPTT SVTPAKPASQ TTIKVKSGDT LWGLSVKYKT TIAQLKSWNH LNSDTIFIGQ NLIVSQSAGS SSSSTGSSSA STSSTSNSSA ASNTSIHKVV KGDTLWGLSQ KSGSPIASIK AWNHLSSDTI LIGQYLRIK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for cell separation during growth.
Gene References into Functions
  1. AcmD, a homolog of the major autolysin AcmA, contributes to cell separation and autolysis. LysMAcmD binds to lactococcal cells preferably at low pH. PMID: 23951292
  2. enzymic N-acetylglucosamine deacetylation by XynD protects peptidoglycan from hydrolysis by the major autolysin AcmA in L. lactis cells, and this leads to decreased cellular autolysis. PMID: 17906127
  3. Growth of L. lactis on galactose changes the composition in the cell wall in such a way that less AcmA is able to bind to the peptidoglycan. PMID: 18539791
  4. Multiple force curves recorded between the LysM tips of the major AcmA and peptidoglycan surfaces yielded a bimodal distribution of binding forces, presumably reflecting the occurrence of one and two LysM-peptidoglycan interactions, respectively. PMID: 18757536
Subcellular Location
Secreted.
Protein Families
Glycosyl hydrolase 73 family
Database Links

KEGG: lla:L68758

STRING: 272623.L68758

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