Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 2-isopropylmalate synthase (leuA), partial

Code CSB-YP809903LOS
MSDS
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Source Yeast
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Code CSB-EP809903LOS
MSDS
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Source E.coli
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Code CSB-EP809903LOS-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP809903LOS
MSDS
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Source Baculovirus
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Code CSB-MP809903LOS
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
leuA
Uniprot No.
Alternative Names
leuA; leuA1; LA_22022-isopropylmalate synthase; EC 2.3.3.13; Alpha-IPM synthase; Alpha-isopropylmalate synthase
Species
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Expression Region
1-501
Target Protein Sequence
MDLKDYVRIF DTTLRDGEQC PGAAMTENEK LEIASQLATM KVDIIEAGFP VSSPVQFQAV ERIARETEGP MIAALARAMK ADIEAASKAL QPAKKRRIHT FIASSPIHMK YKLGKEPKEV LKMAVEAVTL CRQFVDDVEF SPEDATRSEP EFLRELCEAV IAAGATTINI PDTVGYTTPA EYGGLFKFLL SNVRGAEKII FSAHCHNDLG LATANSLAAV QNGARQIECT INGIGERAGN TAMEEVVMAM RTRKDTFGIQ TQIKTEEIAR ASYLVKTITG MLVQPNKAIV GANAFAHESG IHQDGVIKHR ETYEIMKPET VGLSSNRMVL GRHSGRAGFK DRIVKLGFSP QVEELEAAYQ RFLEIADRKK EIYDEDIRAL FSEEARKSTG DRFQLEGFTV STGTKSTPTA GVRILIDGHV REESATGDGP VDAIYKAIQK TTGMDPEVSR LVISPVTEGQ DAMAEASVTL EYKGDRVVGK GSSTDIIEAC SRAYISALNR L
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Does not have citramalate synthase activity.
Protein Families
Alpha-IPM synthase/homocitrate synthase family, LeuA type 1 subfamily
Database Links

KEGG: lil:LA_2202

STRING: 189518.LA_2202

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