Recombinant Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial(Cyp27b1)

Code CSB-EP006406MO
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Cyp27b1
Uniprot No. O35084
Alternative Names Cyp27b1; Cyp27b; Cyp4025-hydroxyvitamin D-1 alpha hydroxylase; mitochondrial; EC 1.14.15.18; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1
Species Mus musculus (Mouse)
Source E.coli
Expression Region 1-507aa
Target Protein Sequence MTQAVKLASRVFHRIHLPLQLDASLGSRGSESVLRSLSDIPGPSTLSFLAELFCKGGLSRLHELQVHGAARYGPIWSGSFGTLRTVYVADPTLVEQLLRQESHCPERCSFSSWAEHRRRHQRACGLLTADGEEWQRLRSLLAPLLLRPQAAAGYAGTLDNVVRDLVRRLRRQRGRGSGLPGLVLDVAGEFYKFGLESIGAVLLGSRLGCLEAEVPPDTETFIHAVGSVFVSTLLTMAMPNWLHHLIPGPWARLCRDWDQMFAFAQRHVELREGEAAMRNQGKPEEDMPSGHHLTHFLFREKVSVQSIVGNVTELLLAGVDTVSNTLSWTLYELSRHPDVQTALHSEITAGTRGSCAHPHGTALSQLPLLKAVIKEVLRLYPVVPGNSRVPDRDIRVGNYVIPQDTLVSLCHYATSRDPTQFPDPNSFNPARWLGEGPTPHPFASLPFGFGKRSCIGRRLAELELQMALSQILTHFEVLPEPGALPIKPMTRTVLVPERSINLQFVDR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 60.2kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Gene References into Functions
  1. Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1alpha-Hydroxylase Expression in Vascular Smooth Muscle Cells PMID: 27074284
  2. These results reveal that differential regulation of Cyp27b1 expression represents a mechanism whereby 1,25(OH)2D3 can fulfill separate functional roles, first in the kidney to control mineral homeostasis and second in extra-renal cells to regulate target genes linked to specific biological responses. PMID: 28808057
  3. The data indicate that abnormal osteoclastogenesis due to the absence of CYP27B1 expression, consistent with the notion that endogenous metabolism of 25-hydroxyvitamin D optimizes osteoclastogenesis and ameliorates the resulting activity of mature osteoclasts. PMID: 26639637
  4. The absence of 25-hydroxyvitamin D3-1alpha-hydroxylase potentiates the suppression of EAE in mice by ultraviolet light. PMID: 27108944
  5. Cyp27b1(-/-) mice exhibited hypocalcemia, growth defects, and skeletogenesis dysfunction, similar to Vdr(-/-) mice, but do not display alopecia PMID: 28025137
  6. Findings demonstrate that in-tumor CYP27B1 1-alpha-hydroxylase activity plays a crucial role in controlling early oncogene-mediated mammary carcinogenesis events, at least in part by modulating tumoral cell NF-kappaB p65 nuclear translocation. PMID: 27119753
  7. the effect of 25-hydroxyvitamin D-1-alpha-hydroxylase on the atherosclerosis disease both in apolipoprotein (apo) E-/- mice and wild-type mice, was investigated. PMID: 28178628
  8. P450C1 alpha deficiency results in abnormal calcium handling and cardiac dysfunction in mice with defective vitamin D signaling. PMID: 25268137
  9. Throughout the male reproductive tract specific bands of CYP27B1 are determined. PMID: 23188491
  10. observed a 3- to 10-fold increase in CYP27B1 mRNA abundance in the lung, spleen, aorta and testis of FGF-23 null/1alpha-Luc mice PMID: 24019880
  11. Data show that the absence of either of the two key hydroxylases, vitamin D 25-hydroxylase (CYP2R1) or vitamin D 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1)neither inhibits nor enhances the development of experimental autoimmune encephalomyelitis (EAE). PMID: 22592802
  12. These results confirm the expression of vitamin D receptor and Cyp27b1 in vivo and suggest a potential role for vitamin D(3) in skeletal muscle regeneration following injury. PMID: 22648952
  13. Bone formation parameters were increased significantly in all pups fed by dams on the rescue diet but were still lower in 1alpha(OH)ase(-/-) pups than in 1alpha(OH)ase(+/-) pups. PMID: 21791625
  14. 1alpha-hydroxylase may have a role in enhanced radiosensitivity PMID: 21343672
  15. regulation of bone CYP27B1 is unique from that in the kidney, and may play an important role in bone formation PMID: 20236619
  16. study shows CYP27B1 can hydroxylate 25-hydroxyvitamin D2 & 25-hydroxyvitamin D3 associated with phospholipid membranes; low activity found at higher membrane levels of 25-hydroxyvitamin D; substrate inhibition may contribute to enzyme activity regulation PMID: 20193763
  17. both 1alphaOHase gene ablation and Pi supplementation inhibit renal calcification in Npt2-/- mice and that 1,25(OH)2D is essential for the development of hypercalciuria and nephrocalcinosis in the mutant strain. PMID: 14656762
  18. leptin suppresses renal gene overexpression for 1 alpha-hydroxylase and 24-hydroxylase and corrects increased serum concentrations of calcium and phosphate in ob/ob mice. PMID: 14657008
  19. 1 alpha-OHase knockout mice fed a normal Ca2+ diet developed severe hypocalcemia, rickets and died with an average life span of 12 +/- 2 weeks PMID: 14717923
  20. 25OHD 1OHase essential for normal epidermal differentiation, most likely by producing vitamin D metabolite, 1,25(OH)(2)D, responsible for inducing proteins regulating calcium levels in epidermis critical for generation and maintenance of barrier. PMID: 15102089
  21. 5' flanking region of the gene directs expression to the proximal convoluted tubules of the kidney PMID: 15691891
  22. study of substrate recognition of CYP27B1 PMID: 15972816
  23. CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D3-1alpha-hydroxylase promoter activity in the skin. PMID: 16371465
  24. These results suggest that adrenodoxin functions as an effector for the oxygen transfer reaction in addition to being an electron donor for CYP27B1. PMID: 16584176
  25. confirm a crucial role for STAT1alpha as well as for C/EBPbeta in the regulation of 1alpha-hydroxylase in monocytes PMID: 17267208
  26. leptin attenuates renal 1alpha-hydroxylase gene expression through ObRb. Furthermore, leptin appears to act indirectly on renal proximal tubules to regulate 1alpha-hydroxylase gene expression. PMID: 17400175
  27. FGF-23 directly regulates renal 1alpha-hydroxylase gene expression via activation of the ERK1/2 signaling pathway. PMID: 17699549
  28. Neither high dietary Ca nor high dietary vitamin D3 is sufficient to fully recover the phenotype of CYP27B1 KO mice. PMID: 18029472
  29. Results demonstrate that the proximal 1.5kb 5' flanking region of the CYP27B1 gene directs the expression of CYP27B1 in a number of known and novel tissues in a specific manner. PMID: 18313834
  30. Lack of Cyp27b1 exacerbates disease in mouse model, suggesting that similar effects may occur with vitamin D deficiency PMID: 18535110
  31. 1alpha-hydroxylase is expressed in adipose tissue and is functional in cultured adipocytes. PMID: 18840526
  32. Results from genetic manipulation of expression of Cyp27b1 in chondrocytes support direct role for locally synthesized 1,25(OH)2D3, acting through the vitamin D receptor, in vascular invasion & osteoclastogenesis during endochondral bone development. PMID: 19477943

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Subcellular Location Mitochondrion membrane
Protein Families Cytochrome P450 family
Tissue Specificity Kidney.
Database Links

KEGG: mmu:13115

STRING: 10090.ENSMUSP00000130005

UniGene: Mm.6216

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