Cyp27b1 Antibody

Code CSB-PA006406ZA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Cyp27b1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
Cyp27b1 antibody; Cyp27b antibody; Cyp4025-hydroxyvitamin D-1 alpha hydroxylase antibody; mitochondrial antibody; EC antibody; 25-OHD-1 alpha-hydroxylase antibody; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase antibody; VD3 1A hydroxylase antibody; Calcidiol 1-monooxygenase antibody; Cytochrome P450 subfamily XXVIIB polypeptide 1 antibody; Cytochrome P450C1 alpha antibody; Cytochrome P450VD1-alpha antibody; Cytochrome p450 27B1 antibody
Raised in
Species Reactivity
Mus musculus
Recombinant Mus musculus Cyp27b1 protein
Immunogen Species
Mus musculus (Mouse)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
Gene References into Functions
  1. Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1alpha-Hydroxylase Expression in Vascular Smooth Muscle Cells PMID: 27074284
  2. These results reveal that differential regulation of Cyp27b1 expression represents a mechanism whereby 1,25(OH)2D3 can fulfill separate functional roles, first in the kidney to control mineral homeostasis and second in extra-renal cells to regulate target genes linked to specific biological responses. PMID: 28808057
  3. The data indicate that abnormal osteoclastogenesis due to the absence of CYP27B1 expression, consistent with the notion that endogenous metabolism of 25-hydroxyvitamin D optimizes osteoclastogenesis and ameliorates the resulting activity of mature osteoclasts. PMID: 26639637
  4. The absence of 25-hydroxyvitamin D3-1alpha-hydroxylase potentiates the suppression of EAE in mice by ultraviolet light. PMID: 27108944
  5. Cyp27b1(-/-) mice exhibited hypocalcemia, growth defects, and skeletogenesis dysfunction, similar to Vdr(-/-) mice, but do not display alopecia PMID: 28025137
  6. Findings demonstrate that in-tumor CYP27B1 1-alpha-hydroxylase activity plays a crucial role in controlling early oncogene-mediated mammary carcinogenesis events, at least in part by modulating tumoral cell NF-kappaB p65 nuclear translocation. PMID: 27119753
  7. the effect of 25-hydroxyvitamin D-1-alpha-hydroxylase on the atherosclerosis disease both in apolipoprotein (apo) E-/- mice and wild-type mice, was investigated. PMID: 28178628
  8. P450C1 alpha deficiency results in abnormal calcium handling and cardiac dysfunction in mice with defective vitamin D signaling. PMID: 25268137
  9. Throughout the male reproductive tract specific bands of CYP27B1 are determined. PMID: 23188491
  10. observed a 3- to 10-fold increase in CYP27B1 mRNA abundance in the lung, spleen, aorta and testis of FGF-23 null/1alpha-Luc mice PMID: 24019880
  11. Data show that the absence of either of the two key hydroxylases, vitamin D 25-hydroxylase (CYP2R1) or vitamin D 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1)neither inhibits nor enhances the development of experimental autoimmune encephalomyelitis (EAE). PMID: 22592802
  12. These results confirm the expression of vitamin D receptor and Cyp27b1 in vivo and suggest a potential role for vitamin D(3) in skeletal muscle regeneration following injury. PMID: 22648952
  13. Bone formation parameters were increased significantly in all pups fed by dams on the rescue diet but were still lower in 1alpha(OH)ase(-/-) pups than in 1alpha(OH)ase(+/-) pups. PMID: 21791625
  14. 1alpha-hydroxylase may have a role in enhanced radiosensitivity PMID: 21343672
  15. regulation of bone CYP27B1 is unique from that in the kidney, and may play an important role in bone formation PMID: 20236619
  16. study shows CYP27B1 can hydroxylate 25-hydroxyvitamin D2 & 25-hydroxyvitamin D3 associated with phospholipid membranes; low activity found at higher membrane levels of 25-hydroxyvitamin D; substrate inhibition may contribute to enzyme activity regulation PMID: 20193763
  17. both 1alphaOHase gene ablation and Pi supplementation inhibit renal calcification in Npt2-/- mice and that 1,25(OH)2D is essential for the development of hypercalciuria and nephrocalcinosis in the mutant strain. PMID: 14656762
  18. leptin suppresses renal gene overexpression for 1 alpha-hydroxylase and 24-hydroxylase and corrects increased serum concentrations of calcium and phosphate in ob/ob mice. PMID: 14657008
  19. 1 alpha-OHase knockout mice fed a normal Ca2+ diet developed severe hypocalcemia, rickets and died with an average life span of 12 +/- 2 weeks PMID: 14717923
  20. 25OHD 1OHase essential for normal epidermal differentiation, most likely by producing vitamin D metabolite, 1,25(OH)(2)D, responsible for inducing proteins regulating calcium levels in epidermis critical for generation and maintenance of barrier. PMID: 15102089
  21. 5' flanking region of the gene directs expression to the proximal convoluted tubules of the kidney PMID: 15691891
  22. study of substrate recognition of CYP27B1 PMID: 15972816
  23. CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D3-1alpha-hydroxylase promoter activity in the skin. PMID: 16371465
  24. These results suggest that adrenodoxin functions as an effector for the oxygen transfer reaction in addition to being an electron donor for CYP27B1. PMID: 16584176
  25. confirm a crucial role for STAT1alpha as well as for C/EBPbeta in the regulation of 1alpha-hydroxylase in monocytes PMID: 17267208
  26. leptin attenuates renal 1alpha-hydroxylase gene expression through ObRb. Furthermore, leptin appears to act indirectly on renal proximal tubules to regulate 1alpha-hydroxylase gene expression. PMID: 17400175
  27. FGF-23 directly regulates renal 1alpha-hydroxylase gene expression via activation of the ERK1/2 signaling pathway. PMID: 17699549
  28. Neither high dietary Ca nor high dietary vitamin D3 is sufficient to fully recover the phenotype of CYP27B1 KO mice. PMID: 18029472
  29. Results demonstrate that the proximal 1.5kb 5' flanking region of the CYP27B1 gene directs the expression of CYP27B1 in a number of known and novel tissues in a specific manner. PMID: 18313834
  30. Lack of Cyp27b1 exacerbates disease in mouse model, suggesting that similar effects may occur with vitamin D deficiency PMID: 18535110
  31. 1alpha-hydroxylase is expressed in adipose tissue and is functional in cultured adipocytes. PMID: 18840526
  32. Results from genetic manipulation of expression of Cyp27b1 in chondrocytes support direct role for locally synthesized 1,25(OH)2D3, acting through the vitamin D receptor, in vascular invasion & osteoclastogenesis during endochondral bone development. PMID: 19477943

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Subcellular Location
Mitochondrion membrane.
Protein Families
Cytochrome P450 family
Tissue Specificity
Database Links
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