Recombinant Mouse 3-mercaptopyruvate sulfurtransferase (Mpst)

Code CSB-YP860793MO
MSDS
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Source Yeast
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Code CSB-EP860793MO
MSDS
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Source E.coli
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Code CSB-EP860793MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP860793MO
MSDS
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Source Baculovirus
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Code CSB-MP860793MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mpst
Uniprot No.
Alternative Names
Mpst; 3-mercaptopyruvate sulfurtransferase; MST; EC 2.8.1.2
Species
Mus musculus (Mouse)
Expression Region
2-297
Target Protein Sequence
AAPQLFRAL VSAQWVAEAL KAPRSSQPLK LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDRCSDHTS PYDHMLPNAT HFADYAGSLG VSAATHVVIY DGSDQGPYSA PRVWWMFRAF GHHSVSLLDG GFRHWLNQNL PISSGKSHSE PAEFSAQLDP SFIKTHEDIL ENLDARRFQV VDARAAGRFQ GTQPEPRDGI EPGHIPGSVN IPFTEFLTNE GLEKSPEEIK RLFKEKKVDL SKPLVATCGS GVTACHVVLG AFLCGKSDVP VYDGSWVEWY MRAQPEHIIS EGRGKTQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Gene References into Functions
  1. Up-regulation of 3-MST was located in living neurons and followed neuronal autophagy after traumatic brain injury. PMID: 27038311
  2. H2S3 and H2S are produced in the brain by 3-mercaptopyruvate sulfurtransferase. PMID: 26437775
  3. behavioral abnormality in MST-KO mice is caused by MST function defects such as an antioxidant insufficiency or a new transducer, H2S (or HS(-)) and/or SOx deficiency. PMID: 23759691
  4. SiRNA silencing of 3-MPST reduced basal bioenergetic parameters and prevented the stimulating effect of 3-MP on mitochondrial bioenergetics. PMID: 23104984
Subcellular Location
Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome.
Tissue Specificity
Expressed in the brain and retina. In the retina, localized to the inner and outer plexiform layer, the inner and outer nuclear layer and the outer segments of photoreceptors. In the brain, localized to neurons of mitral cell layers, glomerular, and exter
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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