Recombinant Mouse ADP-ribosylation factor-related protein 1 (Arfrp1)

Code CSB-YP805360MO
MSDS
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Source Yeast
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Code CSB-EP805360MO
MSDS
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Source E.coli
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Code CSB-EP805360MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805360MO
MSDS
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Source Baculovirus
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Code CSB-MP805360MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arfrp1
Uniprot No.
Alternative Names
Arfrp1ADP-ribosylation factor-related protein 1; ARF-related protein 1
Species
Mus musculus (Mouse)
Expression Region
1-201
Target Protein Sequence
MYTLLSGLYK YMFQKDEYCI LILGLDNAGK TTFLEQSKTR FNKNYKGMSL SKITTTVGLN IGTVDVGKAR LMFWDLGGQE ELQSLWDKYY AECHGVIYVI DSTDEERLSE SKEAFEKVVS SEALDGVPIL VLANKQDVET CLSIPDIKTA FSDCTCKIGR RDCLTQACSA LTGKGVREGI EWMVKCVVRN VHRPPRQRDI T
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.
Gene References into Functions
  1. ARFRP1 and the Golgi apparatus play an important role in lipoprotein maturation in the liver. PMID: 24186947
  2. The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium. PMID: 22505585
  3. data indicate that ARFRP1 in the liver is involved in the regulation of IGF1 secretion and GLUT2 sorting and is thereby essential for normal growth and glycogen storage PMID: 22927645
  4. Arfrp1 appears to be involved in sorting of GLUT4. PMID: 20230794
  5. Lipid droplets of adipocytes of Arfrp1(-/-) mice were smaller and exhibited ultrastructural alterations, such as a disturbed interaction of small lipid particles with larger droplets. PMID: 20038528
  6. ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin. PMID: 18662990
  7. This study provides the first detailed analysis of the regional distribution of ARFRP1 in the mouse brain and a quantitative view of its changes following sleep deprivation. PMID: 18666447

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Subcellular Location
Golgi apparatus. Golgi apparatus, trans-Golgi network.
Protein Families
Small GTPase superfamily, Arf family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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