Recombinant Mouse Achaete-scute homolog 3 (Ascl3)

Code CSB-YP867501MO
MSDS
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Source Yeast
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Code CSB-EP867501MO
MSDS
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Source E.coli
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Code CSB-EP867501MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867501MO
MSDS
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Source Baculovirus
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Code CSB-MP867501MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ascl3
Uniprot No.
Alternative Names
Ascl3; Mash3; Sgn1Achaete-scute homolog 3; ASH-3; mASH-3; mASH3; bHLH transcriptional regulator Sgn-1
Species
Mus musculus (Mouse)
Expression Region
1-174
Target Protein Sequence
MDTRSYPSPP DRLSVFAESA HLPLSRPFYL DPMVTVHLCP ETPVPASYTD ELPLLPFSSD TLIMNNYGDP YPFPFPMPYT NYRRCDYTYG PAFIRKRNER ERQRVKCVNE GYARLRRHLP EDYLEKRLSK VETLRAAIKY ISYLQSLLYP DESETKKNPR TASCGSLDPA LRVI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor. Inhibits myogenesis.
Gene References into Functions
  1. These results demonstrate that Ascl3 marks progenitors that are lineage-committed strictly to microvillar cells and Bowman's glands, and highlight the requirement for these cell types to support OE homeostasis. PMID: 27910949
  2. Ascl3 knockouts develop smaller salivary glands than wild types, but secrete saliva normally. They display a lower level of cell proliferation, consistent with their smaller size. In the absence of Ascl3, the cells maintain their progenitor function. PMID: 21377457
  3. These data demonstrate a progenitor-progeny relationship between duct cells and the acinar cell compartment, and identify a population of multipotent progenitor cells, marked by expression of Ascl3, which is capable of generating both gland cell types. PMID: 18572159
Subcellular Location
Nucleus.
Tissue Specificity
Specifically expressed in the salivary duct cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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