Recombinant Mouse Acidic leucine-rich nuclear phosphoprotein 32 family member A (Anp32a)

Code CSB-YP001822MO
MSDS
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Source Yeast
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Code CSB-EP001822MO
MSDS
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Source E.coli
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Code CSB-EP001822MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001822MO
MSDS
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Source Baculovirus
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Code CSB-MP001822MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Anp32a
Uniprot No.
Alternative Names
Anp32a; Anp32; Lanp; Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; Leucine-rich acidic nuclear protein; LANP; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A
Species
Mus musculus (Mouse)
Expression Region
1-247
Target Protein Sequence
MEMDKRIYLE LRNRTPSDVK ELVLDNCKSI EGKIEGLTDE FEELEFLSTI NVGLTSISNL PKLNKLKKLE LSENRISGDL EVLAEKCPNL KHLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLNA YRENVFKLLP QVMYLDGYDR DNKEAPDSDV EGYVEDDDEE DEDEEEYDEY AQLVEDEEEE DEEEEGEEED VSGEEEEDEE GYNDGEVDDE EDEEEAGEEE GSQKRKREPD DEGEEDD
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription. Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation. In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking. Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition. Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A.
Gene References into Functions
  1. our study reveals that ANP32A dysregulation may be a critical factor contributing to acute megakaryoblastic leukemia PMID: 29269781
  2. We conclude that downregulating ANP32A rescues synaptic plasticity and memory ability by reducing INHAT formation and unmasking histone for hyperacetylation PMID: 28472990
  3. The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development PMID: 25866766
  4. depleting the Leucine-rich Acidic Nuclear Protein (LANP)-an ATXN1 binding inhibitor of histone acetylation-reverses aspects of Spinocerebellar ataxia type 1 neuritic pathology. PMID: 22884877
  5. Regulation of histone acetylation and transcription by nuclear protein pp32, a subunit of the INHAT complex. PMID: 11830591
  6. Results show that leucine-rich acidic nuclear protein (LANP)-null mice are viable and fertile, and point to a functional redundancy of LANP's function, most likely provided by its closely related family members. PMID: 15060138
  7. Results describe the solution structure of the evolutionarily conserved N-terminal leucine-rich repeat (LRR) domain of Anp32a and model its interactions with other proteins. PMID: 18410380
  8. PHAPI/pp32 suppresses tumorigenesis by stimulating apoptosis. PMID: 19121999
  9. LANP directly regulates expression of the neurofilament light chain, an important neuron-specific cytoskeletal gene, by binding to the promoter of this gene and modulating histone acetylation levels PMID: 19136565

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Subcellular Location
Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Shuttles between nucleus and cytoplasm. Translocates to the cytoplasm during the process of neuritogenesis.
Protein Families
ANP32 family
Tissue Specificity
Predominantly expressed in the cerebellum. Expressed also in cortex, lung, skeletal muscle, gastrointestinal tract, spleen, liver and heart.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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