Recombinant Mouse Alpha-tocopherol transfer protein (Ttpa)

Code CSB-YP805352MO
MSDS
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Source Yeast
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Code CSB-EP805352MO
MSDS
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Source E.coli
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Code CSB-EP805352MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805352MO
MSDS
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Source Baculovirus
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Code CSB-MP805352MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ttpa
Uniprot No.
Alternative Names
Ttpa; Alpha-tocopherol transfer protein; Alpha-TTP
Species
Mus musculus (Mouse)
Expression Region
1-278
Target Protein Sequence
MAEMRPGPLV GKQLNELPDH SPLLQPGLAE LRRRVQEAGV PQTPQPLTDA FLLRFLRARD FDLDLAWRLM KNYYKWRAEC PELSADLRPR SILGLLKAGY HGVLRSRDST GSRVLIYRIA YWDPKVFTAY DVFRVSLITS ELIVQEVETQ RNGVKAIFDL EGWQVSHAFQ ITPSVAKKIA AVLTDSFPLK VRGIHLINEP VIFHAVFSMI KPFLTEKIKD RIHLHGNNYK SSMLQHFPDI LPREYGGKEF SMEDICQEWT NFIMKSEDYL SSISETIQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds (+)-alpha-tocopherol, enhances its transfer between separate membranes, and stimulates its release from liver cells. Binds both phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate; the resulting conformation change is important for the release of the bound alpha-tocopherol.
Gene References into Functions
  1. alpha-TTP protects against CQ toxicity by preventing CQ accumulation in acidic organelles through a mechanism distinct from vitamin E transport. PMID: 22147702
  2. Results suggest that lipid peroxidation due to depletion of alpha-tocopherol impairs Abeta clearances from the brain and from the blood, possibly causing increased Abeta accumulation in Ttpa(-/-)APPsw brain and plasma. PMID: 19679659
  3. alpha-tocopherol plays a role in the process of implantation PMID: 12135902
  4. Endogenous TTPA, through the enhancement of MMP 9 expression and proteolytic activation, plays an essential role in the pathogenesis of heparin-produced cerebral hemorrhage. PMID: 14630814
  5. TTP transports alpha-TCP and oral administration of TTP compensates for TTP in TTP-deficient mice PMID: 16257640
  6. These data suggest that in vivo network of alpha-tocopherol (AT), AT-metabolites and ATTP affects the transcription of genes driven by AhR, Nrf2 and NF-kappaB, transcription factor networks. PMID: 17400288
  7. p75(NTR)is up-regulated after tissue injury, blocks fibrinolysis by down-regulating the serine protease, tissue plasminogen activator, and up-regulating PAI-1. PMID: 17576803
  8. changes in hepatic TTP concentrations were minimal in response to dietary vitamin E levels or environmental tobacco smoke-related oxidative stress PMID: 17707975
  9. alpha-tocopherol deficiency decreases oxidative stress and unmasks alpha-tocopherol-dependent regulation of mitochondrial function in the brain PMID: 18180306
  10. Increase expression of tPA implicate fibrinolysis in ALD progression. PMID: 18289715
  11. The results show that both gender and the hepatic alpha-TTP, but not brain alpha-TTP gene expression are important in determining alpha-T concentrations within the brain. PMID: 18299118

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Subcellular Location
Cytoplasm.
Database Links
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