Recombinant Mouse Angiomotin-like protein 2 (Amotl2)

Code CSB-YP809099MO
MSDS
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Source Yeast
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Code CSB-EP809099MO
MSDS
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Source E.coli
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Code CSB-EP809099MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP809099MO
MSDS
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Source Baculovirus
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Code CSB-MP809099MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Amotl2
Uniprot No.
Alternative Names
Amotl2Angiomotin-like protein 2
Species
Mus musculus (Mouse)
Expression Region
1-772
Target Protein Sequence
MRTLEDSSGT VLHRLIQEQL RYGNLTETRT LLAIQQQALR GGAGAGGTGS PQASLEIGAP EDSQVLQQAT RQEPQGQEHQ GGETHLAENR LYRLCPQPSK GEELPTYEEA KAHSQYYAAQ QAGSRPHVGD RDPRGGVSGG GRRQDEALRE LRHGHVRSLS ERLLQLSLER NGARVPSHMS SSHSFPQLAR SQQGPQPRGP PAEGPEPRGP PPQYPHAVMA QETAAVTDPR YRPRSSPHFQ HAEVRILQAQ VPPVFLQQQQ YQYLPQPQEH SPPLHPAALG HGPPSSFGPP AVEGPPSAQA TLGSAHLAQM ETVLRENARL QRDNERLQRE LESTSEKAGR IEKLENEIQR LSEAHESLMR TSSKREALEK TMRNKMDGEM RRLQDFNRDL RERLESANRH LASKTQEAQA GSQDMVAKLL AQSYEQQQEQ EKLEREMALL RGAIEDQRRH AELLEQALGN AQSRAARAEE ELRKKQAYVE KVERLQQALG QLQAACEKRE QLELRLRTRL EQELKALRAQ QRQTGTLAGG GGSHGGSAEL SALRLSEQLR EKEEQILALE ADMTKWEQKY LEERAMRQFA MDAAATAAAQ RDTTLIRHSP QPSPSSSFNE GLLPGNHRHQ EMESRLKVLH AQILEKDAVI KVLQQRSRKD PGKATQGTLR PAKSVPSIFA AAVGTQGWQG LVSSERQTDA RPAGDRVPAE EPPATAPLPA HTKHGSRDGS TQTDGPADNT SACLASEPDG LLGCNSSQRT PSLDSIAATR VQDLSDMVEI LI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC.
Gene References into Functions
  1. AmotL2 disrupts apical-basal cell polarity and promotes tumor invasion. PMID: 25080976
  2. Amotl2 plays a pivotal role in polarity, migration and proliferation of angiogenic endothelial cells PMID: 21937427
Subcellular Location
Recycling endosome.
Protein Families
Angiomotin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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