Recombinant Mouse Anoctamin-1 (Ano1), partial

1. Bile acids stimulate Cl(-) secretion in biliary cells through activation of membrane TMEM16A channels in a process regulated by extracellular ATP and [Ca(2+) ]i . PMID: 29360145
2. TMEM16A modulates arterial contractility, at least in part, indirectly via regulation of CACNA1C expression. PMID: 29438598
3. the glutamic acids (E) at 143 and 705 residues in ANO1 are critical for modulation of Ca2 and/or heat responses. PMID: 29335069
4. anoctamin-1 is involved in psychiatric behavior because of its role in the regulation of synaptic transmission in presynaptic terminals PMID: 29928889
5. de novo atomic structures of the transmembrane domains of mouse TMEM16A in nanodiscs and in lauryl maltose neopentyl glycol as determined by single-particle electron cryo-microscopy PMID: 29236684
6. cryo-EM structures of mouse TMEM16A at high resolution in the presence and absence of Ca(2+) PMID: 29236691
7. The protein shows a similar organization to nhTMEM16, except for changes at the site of catalysis. There, the conformation of transmembrane helices constituting a membrane-spanning furrow that provides a path for lipids in scramblases has changed to form an enclosed aqueous pore that is largely shielded from the membrane. PMID: 28561733
8. ANO1 immunoreactivity was observed in the presynaptic terminals of various retinal neurons, including photoreceptors PMID: 23840801
9. In this work we demonstrate that increasing the extracellular proton concentration from 10-10 to 10-5.5 m enabled TMEM16A activation. Furthermore, we show that this effect is voltage independent, caused by changes in the apparent open probability of the channel, and due to protonation of extracellular residue E623 PMID: 27859335
10. The present study shows that ANO1 and CavL play a central role in the generation of slow waves, phasic contractions and tone in the internal anal sphincter and that this pathway can occur in the absence of stretch PMID: 28054347
11. findings demonstrate that Ca2+ influx via store-operated CRAC channels is essential for CaCC activation, chloride secretion, and sweat production in humans and mice. PMID: 27721237
12. We knocked out Ano1 to varying degrees in interstitial cells of Cajal (ICC) of adult mice. Partial knockout of Ano1 shortened the widths of electrical slow waves and Ca(2+) transients in myenteric ICC but Ca(2+) transient synchronicity was preserved. Near-complete knockout was necessary for transient desynchronization and loss of slow waves, indicating a large functional reserve of Ano1 in ICC. PMID: 27979828
13. Phosphorylation levels of P38 and JNK in siRNA-TMEM16A group were lower than that of the Model group. Thus, TMEM16A is one of the critical components of a signal transduction pathway that links renal injury to podocyte apoptosis in DN. PMID: 28392397
14. To determine the role of ANO1 in physiological functions. PMID: 27413167
15. ANO1/TMEM16A is a significant pathway in pancreatic acinar cells for HCO3 (-) secretion into the lumen. PMID: 27510033
16. TMEM16A is a positive regulator of endothelial reactive oxygen species generation via Nox2-containing NADPH oxidase, which induces endothelial dysfunction and hypertension. PMID: 28320851
17. Data indicate that anoctamin channel proteins ANO1 and ANO2 are expressed in the cerebellum. PMID: 26558388
18. Ano1 may play a role in the pathophysiological processes during ischaemia in heart, and therefore, Ano1 might be a potential therapeutic target to prevent ischaemic damage PMID: 24758567
19. Thegenetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. PMID: 26067252
20. Suggest Ca(2+)-dependent push-pull conformational change as a key electromechanical movement for gating the ANO1 channel. PMID: 25231974
21. These results indicate ANO1 channels are involved with TRPV1 actions in sensory neurons and inhibition of ANO1 could be a novel means of inducing analgesia. PMID: 26364309
22. Basolateral Ano1 supports CCH-induced Ca2+ signaling and secretion via luminal CFTR. PMID: 24974903
23. By inhibiting TRPM7 and ANO1 via opioid receptor signaling pathways. PMID: 25967872
24. TMEM16A is an essential component of Ca(2+)-activated Cl(-) currents in mouse vomeronasal sensory neurons. PMID: 25779870
25. conclude that ANO1 encodes Ca(2+)-activated chloride (Cl(-)) channel and plays a significant role in the phase 1 repolarization of action potentials PMID: 24962810
26. TMEM16A and myocardin form a positive feedback loop that is disrupted by KLF5 during Ang II-induced vascular remodeling. PMID: 26077572
27. ANO1 is thus mainly localized at presynaptic terminals in various brain regions, specifically in two auditory brainstem nuclei. PMID: 24853671
28. These results indicate that TRPV1-ANO1 interaction is a significant pain-enhancing mechanism in the peripheral nervous system. PMID: 25848051
29. WT TMEM16A but not mutants with alanine substitution at four basic residues exhibited a clear decline in the preference for larger anions as intracellular calcium was increased. PMID: 25733897
30. TMEM16a is a Ca(2+) -activated Cl(-) channel expressed in the collecting ducts. PMID: 24913262
31. results using acinar-specific Tmem16A(-/-) mice identify Tmem16A as the Cl(-) channel essential for muscarinic, Ca(2+)-dependent fluid secretion in adult mouse salivary glands PMID: 25646474
32. Null deletion of TMEM16A reduced the Ca(2+)-activated Cl(-) currents and action potential firing in spiral ganglion neurons. PMID: 25675481
33. Ano1 has a role in proximal tubular proton secretion and protein reabsorption. PMID: 24476694
34. Calcium-activated chloride secretion by anoctamin 1 appears to be a crucial component of renal cyst growth. PMID: 24152967
35. Selective antagonism of ANO1 promotes relaxation of spontaneous myometrial contractions. It inhibits both agonist-induced and spontaneous transient inward currents and abolishes G-protein coupled receptor mediated elevations in intracellular calcium. PMID: 24928056
36. The Ca(2+)-activated Cl ((-)) channel anoctamin-1 (ANO1/TMEM16A) is located in the primary cilium and that blocking its channel function pharmacologically or knocking it down with short hairpin RNA interferes with ciliogenesis. PMID: 24694595
37. Data suggest different functional roles for TMEM16A and TMEM16B in the developing as well as in the postnatal olfactory epithelium PMID: 24318978
38. TMEM16A is a major component of Ca(2+)-activated Cl(-) channel (CaCC) conductance in murine portal vein smooth muscle cells (mPVSMCs). PMID: 24770592
39. Anoctamin (Ano)1 activation by the divalent calcium (Ca)2+ channel occurs by direct binding of Ca2+ to Ano1. The activation of Ano1 by Ca2+ is not mediated by calmodulin (CaM). PMID: 24420770
40. ANO1 is also capable of augmenting the excitability of dorsal-root ganglion neurons under inflammatory or neuropathic conditions. PMID: 24450308
41. Ano1 is expressed in ovarian granulosa cells and downregulates estrogen production, possibly through the MEK-ERK signaling cascade. PMID: 24823391
42. findings show TMEM16A and TMEM16F have an important function in cell migration-TMEM16A in directional migration, TMEM16F in determination of speed of migration; conclude that TMEM16A and TMEM16F channels have a distinct impact on steering and motor mechanisms of migrating ELA cells PMID: 23832500
43. TMEM16A and -16F use a similar mechanism for sorting to plasma membrane and protein stabilization, but their functional domains significantly differ PMID: 24478309
44. TMEM16A plays a general role in arteriolar and capillary blood flow and may be involved in reducing hypertension PMID: 24401273
45. In conclusion, alternatively spliced variants of TMEM16A abc and acd in mPVSMCs are two major molecular entities of CaCC and may form hetero-/homo-dimers to be functional as CaCC in the regulation of membrane excitability and contractility in mPVSMCs. PMID: 24321548
46. ANO1 decreased activity and expression is associated with abnormal cystic fibrosis airway epithelial repair. PMID: 24080196
47. The putative pore-loop of TMEM16A and TMEM16B channels affects channel density in cell membranes. PMID: 23613533
48. These data provide evidence for a functional role of TMEM16A in the vasoconstrictor responses of murine thoracic aorta and mesenteric artery as well as human resistance vessels. PMID: 22946562
49. 16A Ca(2+)-activated Cl-channels are inferred to be involved in airway hyperresponsiveness. PMID: 23239156
50. HCO3- permeability of ANO1 can be dynamically modulated by Ca2+/calmodulin. PMID: 23248295

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KEGG: mmu:101772

STRING: 10090.ENSMUSP00000113899

UniGene: Mm.26700