Recombinant Mouse Atrophin-1 (Atn1), partial

Code CSB-YP002305MO
MSDS
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Source Yeast
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Code CSB-EP002305MO
MSDS
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Source E.coli
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Code CSB-EP002305MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002305MO
MSDS
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Source Baculovirus
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Code CSB-MP002305MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Atn1
Uniprot No.
Alternative Names
Atn1; DrplaAtrophin-1; Dentatorubral-pallidoluysian atrophy protein homolog
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional corepressor. Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of the poly-Q repeats. Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation.
Gene References into Functions
  1. These results support the model that poly-Q expanded Atrophin-1 proteins cause dentatorubral-pallidoluysian atrophy in a manner independent of any functional interaction with wild-type Atrophin-1 proteins. PMID: 19681162
  2. mutant atropphin with polyglutamine expansion does not simply function in a dominant negative manner PMID: 16407196
  3. TLX interacts with atrophin1 (Atn1), a corepressor that is involved in human neurodegenerative dentatorubral-pallidoluysian atrophy (DRPLA) and that is essential for development of multiple tissues. PMID: 16702404
  4. Atrophin-1 and the short form of Atrophin-2 can act as potent and evolutionarily conserved transcriptional activators. PMID: 17150957
  5. Fat1 and Atrs 1,2 act in concert after vascular injury but show further that distinct Atr isoforms have disparate effects on VSMC directional migration. PMID: 19131340

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Subcellular Location
Cytoplasm, perinuclear region. Cell junction. Nucleus.
Tissue Specificity
Widely expressed. Most abundant in the brain.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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