Recombinant Mouse Bloom syndrome protein homolog (Blm), partial

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Code CSB-EP002715MO
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Greater than 90% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Alternative Names
BlmBloom syndrome protein homolog; mBLM; EC; RecQ helicase homolog
Mus musculus (Mouse)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
Protein Length
Tag Info
N-terminal 10xHis-tagged and C-terminal MYC-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction. Recruited by the KHDC3-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.
Gene References into Functions
  1. In the absence of BLM, sister chromatid exchange events do not occur randomly throughout the genome but are strikingly enriched at coding regions, specifically at sites of guanine quadruplex motifs in transcribed genes. PMID: 29348659
  2. The anti-recombinase activity of BLM is of general importance for normal retention of RAD51 at DNA double strand break sites and regulation of homologous recombination. PMID: 28912125
  3. BLM helicase facilites telomere replication by resolving G4 structures formed during copying of the G-rich strand by leading strand synthesis. PMID: 26195664
  4. Data suggest that BLM suppresses homologous recombination, while full-length BRCA1 promotes this process. PMID: 21709021
  5. Although interhomolog recombination is slightly decreased in the absence of BLM, loss of heterozygosity is increased by fivefold or more, implying significantly increased interhomolog crossing over. PMID: 21730139
  6. Blm has roles both upstream and downstream of the Rad54 protein, a core HR factor. Disruption of Rad54 in the Blm-mutant background reduced the elevated level of gene targeting and of sister chromatid exchanges. PMID: 20531307
  7. the characterization of Hprt mutations in vivo in Blm hypomorphic mice PMID: 20299287
  8. there was no dramatic qualititative difference in the basal cell carcionoma or rhabdomyosarcoma tumors associated with the mutant Blm genotype PMID: 19995795
  9. Data show that BLM-deficient mouse and human cells suppress homeologous recombination to a similar extent as wild-type cells. PMID: 20154148
  10. observations indicate that Blm is a modifier of tumor formation in the mouse and that Blm haploinsufficiency is associated with tumor predisposition PMID: 12242442
  11. Bone marrow cells from mice heterozygous for BLM mutation, BLM(Cin/+), transfected with BCR/ABL display increased sensitivity to cisplatin. PMID: 15750625
  12. Blm gene mutation produces this instability, strengthening a role for CIN in the development of human cancer. PMID: 16914751
  13. Blm serves a vital role for development, maintenance, and function of T lymphocytes, suggesting a basis for the immune deficiency in Bloom's syndrome. PMID: 17210642
  14. BLM, the RecQ DNA helicase mutated in Bloom syndrome, is preferentially modified by SUMO-2/3 both in vitro and in vivo PMID: 18708356
  15. A vital role is reported for RecQ family helicase Blm in all major aspects of the B cell life cycle, manifest in disturbed B cell immunity and tumor development in its absence. PMID: 19109166
  16. The sister chromatid exchange events stimulated by Tim reduction were largely mediated via a Brca2/Rad51-dependent mechanism and were additively increased by deletion of the Blm helicase. PMID: 19112184

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Subcellular Location
Protein Families
Helicase family, RecQ subfamily
Tissue Specificity
Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen. Expressed in bone marrow.
Database Links
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