Recombinant Mouse CMRF35-like molecule 8 (Cd300a), partial

Code CSB-YP747575MO
MSDS
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Source Yeast
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Code CSB-EP747575MO
MSDS
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Source E.coli
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Code CSB-EP747575MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP747575MO
MSDS
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Source Baculovirus
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Code CSB-MP747575MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cd300a
Uniprot No.
Alternative Names
Cd300a; Clm8; Lmir1; Mcpir1CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; Leukocyte mono-Ig-like receptor 1; Mast cell-derived paired immunoglobulin-like receptor 1; Myeloid-associated immunoglobulin-like receptor 1; MAIR-1; MAIR-I; CD antigen CD300a
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6.
Gene References into Functions
  1. The results indicate that CD300a on MCs and DCs regulates neutrophil recruitment into the peritoneal cavity after cecal ligation and puncture (CLP). PMID: 27836913
  2. Epithelial apoptotic cells negatively regulated commensal-mediated signaling in dendritic cells via CD300a for Treg cell proliferation. PMID: 26855029
  3. Data show that peroxisome proliferator activator receptor delta (PPARdelta/beta) directly regulates CD300a protein in macrophages. PMID: 24958459
  4. These results suggest that CD300a is involved in aluminium-induced Th2 skewing. PMID: 25911756
  5. study demonstrates a new tumor immune evasion mechanism that is mediated through the interaction between phosphatidylserine and CD300a and we suggest that CD300c, similarly to CD300a, also interacts with phosphatidylserine. PMID: 23640773
  6. CD300a-deficient mice showed increased neutrophil recruitment and improved bacterial clearance in the peritoneal cavity. PMID: 22826299
  7. These results indicated that CD300a is a new phosphatidylserine receptor. PMID: 22185693
  8. in a murine model of allergic peritonitis, neutralization of the murine homolog of IRp60, LMIR1, led to a significantly augmented release of inflammatory mediators and eosinophilic infiltration. PMID: 16339535

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
CD300 family
Tissue Specificity
Present on the surface of the majority of myeloid cells and a subset of B-cells. Present on the surface of NK cells after IL-12 stimulation.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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